{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:44:09Z","timestamp":1777445049288,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,2,5]],"date-time":"2002-02-05T00:00:00Z","timestamp":1012867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human herpesviruses can infect the eye and be excreted subsequently in tears. The aim of the present study was to use a multiplex PCR to detect herpesviruses (HSV\u20101, \u20102, VZV, CMV, EBV, HHV\u20106) in tears from normal subjects and from patients with pathological conditions (acute herpes, zoster, papillary conjunctivitis, and dry eye). Schirmer test strips were used to collect tear fluid from 93 patients, sampling both eyes. DNA was then extracted from the 186 samples by chromatography, and viral DNA amplified using a commercialised multiplex \u201cstair primer\u201d method. Thirty\u2010four samples (18.3%) contained Taq inhibitors. The multiplex test gave positive results for HSV and VZV in tear fluid from patients with acute dendritic keratitis (3 patients) and acute ocular zoster (4 patients) and was, therefore, considered effective in testing samples from patients with acute lesions. HSV\u20101 and HSV\u20102 were found in two samples from patients with metaherpetic corneal scarring. Among 28 cases of dry eye, two were positive for HHV\u20106, the latter being associated with EBV in one patient. HHV\u20106 was also found in 4 out of 54 cases of papillary conjunctivitis. This raised occurrence of HHV\u20106 in dry eye or papillary conjunctivitis, suggests new clinical patterns for HHV\u20106 latency or reactivation. Detection of EBV in 1 out of 80 healthy eyes confirms previous evidence that lacrimal glands constitute potentially a site for latent\u2010phase EBV. J. Med. Virol. 66:506\u2013511, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.2173","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T04:51:41Z","timestamp":1030251101000},"page":"506-511","source":"Crossref","is-referenced-by-count":28,"title":["Multiplex detection of herpesviruses in tear fluid using the \u201cstair primers\u201d pcr method: Prospective study of 93 patients"],"prefix":"10.1002","volume":"66","author":[{"given":"Pierre\u2010Yves","family":"Robert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Traccard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"Adenis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Denis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Ranger\u2010Rogez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,2,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9071(200011)62:3<349::AID-JMV7>3.0.CO;2-L"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(95)01967-7"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01682169"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.83.8.957"},{"key":"e_1_2_1_6_1","first-page":"390","article-title":"Detection of herpes simplex virus DNA in tear fluid of stromal herpetic keratitis patients by nested polymerase chain reaction","volume":"40","author":"Kudo E","year":"1996","journal-title":"Jap J Ophthalmol"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199903000-00001"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199703)51:3<231::AID-JMV15>3.0.CO;2-N"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.950-953.1999"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(90)32476-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(90)32595-2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1128\/JCM.38.7.2753-2755.2000","article-title":"Detection of human herpesvirus 6 and varicella\u2010zoster virus in tear fluid of patients with Bell's palsy by PCR","volume":"38","author":"Pitkaranta A","year":"2000","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689209001785"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1654(1998100)8:4<187::AID-RMV221>3.0.CO;2-S"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/313932"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2714-2717.1998"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)70497-0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(98)97042-7"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.10.2643-2646.1995"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)73071-5"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.2173","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.2173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T10:08:56Z","timestamp":1700388536000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.2173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,5]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1002\/jmv.2173"],"URL":"https:\/\/doi.org\/10.1002\/jmv.2173","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,5]]}}}