{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:48:20Z","timestamp":1790106500294,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,2,5]],"date-time":"2002-02-05T00:00:00Z","timestamp":1012867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Public health laboratories require rapid diagnosis of dengue outbreaks for application of measures such as vector control. We have developed a rapid single fluorogenic probe\u2010based polymerase chain reaction assay for the detection of all four dengue serotypes (FUDRT\u2010PCR). The method employs primers and probe that are complementary to the evolutionarily conserved 3\u2032 untranslated region of the dengue genome. The assay detected viral RNA of strains of all four dengue serotypes but not of the flaviviruses Japanese encephalitis virus, Murray Valley encephalitis virus, Kunjin, Stratford, West Nile, Alfuy or Yellow fever. When compared to an existing nested\u2010PCR assay for the detection of dengue on clinical samples, FUDRT\u2010PCR detected dengue 1 (100%, <jats:italic>n<\/jats:italic>\u2009=\u200914), dengue 2 (85%, <jats:italic>n<\/jats:italic>\u2009=\u200913), dengue 3 (64%, <jats:italic>n<\/jats:italic>\u2009=\u200914) and dengue 4 (100%, <jats:italic>n<\/jats:italic>\u2009=\u20093) with the indicated sensitivities. FUDRT\u2010PCR enables diagnosis of acute dengue infection in four hours from sample receipt. In addition, a single\u2010test procedure should result in a reduction in the number of tests performed with considerable cost savings for diagnostic laboratories. J. Med. Virol. 66:524\u2013528, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.2176","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T04:51:41Z","timestamp":1030251101000},"page":"524-528","source":"Crossref","is-referenced-by-count":88,"title":["Single rapid TaqMan fluorogenic probe based PCR assay that detects all four dengue serotypes"],"prefix":"10.1002","volume":"66","author":[{"given":"David","family":"Warrilow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A.","family":"Northill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa","family":"Pyke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg 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