{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:47:34Z","timestamp":1700668054053},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,5,25]],"date-time":"2010-05-25T00:00:00Z","timestamp":1274745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human polyomaviru<jats:italic>s<\/jats:italic> JC virus (JCV) infects 70\u201380% of humans and establishes latent infection in the kidney. In immunosuppressed patients, JCV reactivates and causes a fatal and progressive neurological disease known as progressive multifocal leukoencephalopathy (PML). Over the past three decades, PML has become an important neurological complication in acquired immunodeficiency syndrome (AIDS) patients. Recently, it was reported that patients treated with therapeutics that target the integrin receptor very late antigen (VLA)\u20104 are at increased risk of developing PML. However, the relationship between Natalizumab and this unexpected onset of PML has yet to be elucidated. Here, we investi\u2010gated the effect of Natalizumab on the growth of JCV in the permissive human neural cell line IMR\u201032 following viral inoculation. Natalizumab had no effect either on the expression levels of viral proteins as determined by immunoblot analysis using specific antibodies or on the hemagglutination activity of cellular lysates from infected cells. These results suggest that there is no direct effect of Natalizumab on JCV infectivity in IMR\u201032 cells in vitro. J. Med. Virol. 82: 1229\u20131235, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21805","type":"journal-article","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T14:50:16Z","timestamp":1274885416000},"page":"1229-1235","source":"Crossref","is-referenced-by-count":2,"title":["Natalizumab has no direct biological effect on JC virus infectivity in permissive human neural cell 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