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The purpose of this study was to compare two methods [endpoint titration RT\u2010PCR and quantitative RT\u2010PCR (RT\u2010qPCR)] with respect to quantification of Norwalk virus (NV) in inocula made from purified stock suspensions of human fecal specimens. A full\u2010length NV RNA transcript was developed to facilitate quantification using RT\u2010qPCR and provided log linear detection in the range of 49\u20134.9\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> genome equivalent copies (GEC) per reaction. Endpoint titration RT\u2010PCR was used to estimate PCR detection units, and RT\u2010qPCR was used to estimate genome copies in two NV inocula (8fIIa and 8fIIb) used in previous human challenge studies. Overall, RT\u2010qPCR was 1.1\u20131.6\u2009log<jats:sub>10<\/jats:sub> more sensitive (lower detection limit) than endpoint titration RT\u2010PCR when the same RNA release method, PCR primers and thermocycle program were used. These findings have important implications for many experimental interpretations, not the least of which is estimating the median infectious dose in human challenge studies. J. Med. Virol. 82:1612\u20131616, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21851","type":"journal-article","created":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T15:48:43Z","timestamp":1279208923000},"page":"1612-1616","source":"Crossref","is-referenced-by-count":23,"title":["Quantification of Norwalk virus inocula: Comparison of endpoint titration and real\u2010time reverse transcription\u2010PCR 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