{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T02:24:15Z","timestamp":1781922255027,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T00:00:00Z","timestamp":1279152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Laboratory testing for Human T\u2010lymphotropic Virus type 1 and 2 (HTLV\u20101 and \u20102) infections has become routine in blood transfusion, tissue transplantation and clinical diagnoses in many countries worldwide. Screening is usually based on the detection of antibodies to HTLV\u20101 and\/ or \u20102. The number of commercially available assays is limited, and among them, ELISA tests based on microtiter format are most commonly used. Recently, the new <jats:italic>r<\/jats:italic>HTLV\u2010I\/II assay (Abbott Laboratories, Abbott Park, IL) was released; this assay was developed for an automatic large\u2010scale screening platform. This assay was evaluated using pre\u2010characterized serum panels and routine samples from the clinical laboratory. The sensitivity was 100% for HTLV\u20101 and \u20102 (99\/99 and 42\/42, respectively, including one sample that was dually reactive, HTLV\u20101\u2009+\u20092). To test assay specificity, panels of blood donor sera, specimens from patients with autoimmune diseases and some viral infections were used. False\u2010reactive samples from previous HTLV diagnoses were also included. With these panels, the specificity was 99.4% (619\/623). However, the four false\u2010reactive samples all belonged to the group of samples that were previously considered as false\u2010reactive for HTLV\u2010antibodies. All other samples were negative by the <jats:italic>r<\/jats:italic>HTLV\u2010I\/II assay, and thus 100% specificity was obtained. The 1,412 samples tested in the clinic by this assay in routine use were all negative (100% specificity). Taken together, the overall specificity was 99.8%. The assay was sensitive, specific and appropriate for the large\u2010scale screening of samples for HTLV\u20101\/2 antibodies. J. Med. Virol. 82:1606\u20131611, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21867","type":"journal-article","created":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T15:48:43Z","timestamp":1279208923000},"page":"1606-1611","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of a new screening assay for HTLV\u20101 and \u20102 antibodies for large\u2010scale use"],"prefix":"10.1002","volume":"82","author":[{"given":"Kerstin","family":"Malm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torbj\u00f6rn","family":"Kjerstadius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f6ren","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365549509047065"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199708010-00014"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39080845.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(01)00289-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.09.012"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-2-30"},{"key":"e_1_2_5_8_1","first-page":"775","article-title":"Feasibility of human T\u2010lymphotropic virus type I screening using pooled sera","volume":"101","author":"Chang CS","year":"2002","journal-title":"J Formos Med Assoc"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199600001-00031"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.1.105"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01603-08"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2009.00907.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20268"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199508000-00014"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21083"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00690-06"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00114.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70081-6"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501734102"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21867","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21867","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:13:48Z","timestamp":1700666028000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,15]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/jmv.21867"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21867","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,15]]}}}