{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:00:27Z","timestamp":1773860427719,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Human herpesvirus 6<\/jats:italic> (HHV\u20106) and <jats:italic>7<\/jats:italic> (HHV\u20107) are common opportunistic agents in immunocompromised hosts, although infection with HHV\u20106 and HHV\u20107 can also be observed in immunocompetent hosts. Despite similar biology and epidemiology, this study evaluated differences in the IgG subclass distribution associated with HHV\u20106 and HHV\u20107 in seropositive, healthy persons. The identified subclasses were also compared with the detection of HHV\u20106 and HHV\u20107 DNA. For these assays, sera, plasma, and saliva samples were obtained from 40 healthy blood donors in Argentina who were seropositive for both HHV\u20106 and HHV\u20107. HHV\u20106 and HHV\u20107 DNA were detected in saliva and plasma samples using nested PCR, and specific IgG subclasses were determined using immunofluorescent assays of sera samples. HHV\u20107 DNA was detected in 90% of all plasma samples and in 100% of saliva samples. In contrast, HHV\u20106 DNA was not detected in any of the plasma samples, and it was detected in only 6 of 40 saliva samples. Determination of IgG subclass distributions showed that HHV\u20106 was restricted to IgG1, whereas HHV\u20107 IgG subclasses included two groups, one restricted only to IgG1 and the other to IgG1 and IgG3. These results demonstrate the differences between HHV\u20106 and HHV\u20107 DNA range detection in saliva and plasma samples, as well as the IgG subclass patterns for each virus type, in healthy persons in Argentina. J. Med. Virol. 82:1679\u20131683, 2010. 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21880","type":"journal-article","created":{"date-parts":[[2010,9,14]],"date-time":"2010-09-14T05:48:27Z","timestamp":1284443307000},"page":"1679-1683","source":"Crossref","is-referenced-by-count":11,"title":["IgG subclasses and DNA detection of HHV\u20106 and HHV\u20107 in healthy individuals"],"prefix":"10.1002","volume":"82","author":[{"given":"Patricia","family":"Biganzoli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Ferreyra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Sicilia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Carabajal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Frattari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Littvik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Nates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Pavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"3223","DOI":"10.1128\/jcm.34.12.3223-3225.1996","article-title":"Presence of human herpesvirus 6 variants A and B in saliva and peripheral blood mononuclear cells of healthy adults","volume":"34","author":"Aberle SW","year":"1996","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.12.019"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1157\/13051907"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10552"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/367677"},{"key":"e_1_2_6_7_1","first-page":"1222","article-title":"Detection of DNA of lymphotropic herpesviruses in plasma of human immunodeficiency virus\u2010infected patients: Frequency and clinical significance","volume":"9","author":"Broccolo F","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2005.07.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.05.013"},{"key":"e_1_2_6_10_1","first-page":"173","article-title":"Molecular biology and clinical associations of Roseoloviruses human herpesvirus 6 and human herpesvirus 7","volume":"30","author":"Caselli E","year":"2007","journal-title":"New Microbiol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800584"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03165124"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01650204"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21702"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.35.6.1600-1603.1997"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2002.tb02683.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.170-174.2001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200206000-00016"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1128\/jcm.27.1.139-144.1989","article-title":"Detection by enzyme\u2010linked immunosorbent assay of specific immunoglobulin G isotypes in primary and established cytomegalovirus infections","volume":"27","author":"Joassin L","year":"1989","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9788"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.2.396"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002770100359"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073205"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1128\/jcm.21.1.117-121.1985","article-title":"Subclass distribution of rubella virus\u2010specific immunoglobulin G","volume":"21","author":"Linde GA","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803008823"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20337"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.2.273"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1128\/jcm.20.1.94-98.1984","article-title":"Virus\u2010specific immunoglobulin G subclasses in herpes simplex and varicella\u2010zoster virus infections","volume":"20","author":"Sundqvist VA","year":"1984","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.11.008"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.5.3206-3209.1992"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.11.6260-6265.1991"},{"key":"e_1_2_6_32_1","first-page":"393","article-title":"The study of IgG subclass profiles of anti\u2010HBC in populations with different status of HBV infection","volume":"2","author":"Yang YY","year":"2005","journal-title":"Cell Mol Immunol"},{"key":"e_1_2_6_33_1","first-page":"367","article-title":"Isolation of Nanjing local strains of HHV\u20107 and their biological and immunological characteristics","volume":"1","author":"Yao K","year":"2004","journal-title":"Cell Mol Immunol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890410412"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.1.65"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21880","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T21:17:55Z","timestamp":1696886275000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1002\/jmv.21880"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21880","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}