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Ninety\u2010five women with HPV 52 infection (33 transient infections, 17 cervical intraepithelial neoplasia grade II, 15 cervical intraepithelial neoplasia grade III, and 30 invasive cervical cancers) were examined for T\u2010cell responses using interferon\u2010\u03b3 enzyme\u2010linked immunospot (IFN\u2010\u03b3 ELISPOT) assay. Of the 29 peptides (13 L1, 10 E6, and 6 E7) screened positive by an in vitro peptide\u2010binding assay, 14 were positive by the IFN\u2010\u03b3 ELISPOT assay. Positive epitopes for HLA A11 were located at amino acid positions 103\u2013111, 332\u2013340, 342\u2013350, and 373\u2013381 of the L1 protein; and at 27\u201335 and 86\u201394 of the E6 protein; and at 1\u20139 and 27\u201335 of the E7 protein. A24\u2010specific epitopes included 60\u201368 and 98\u2013106 of the L1 protein, 42\u201350 and 59\u201367 of the E6 protein, and 24\u201332 of the E7 protein. Only one epitope (99\u2013107) of the E6 protein showed positive responses for HLA A2 subjects. Overall, T\u2010cell responses against L1 were observed mainly in subjects who had cleared infection; whereas responses against E6 and E7 were confined mainly to subjects who had developed cervical neoplasia. The proportion of subjects showing detectable T\u2010cell responses was low across all grades of cervical neoplasia suggesting that immune evasion mechanisms had set on early in the course of disease progression. This study provides the first set of T\u2010cell epitopes mapped for HPV 52, which can be considered for further evaluation as targets for immunotherapy. J. Med. Virol. 83:1023\u20131030, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21889","type":"journal-article","created":{"date-parts":[[2011,4,18]],"date-time":"2011-04-18T16:29:55Z","timestamp":1303144195000},"page":"1023-1030","source":"Crossref","is-referenced-by-count":11,"title":["T\u2010cell response to human papillomavirus type 52 L1, E6, and E7 peptides in women with transient infection, cervical intraepithelial neoplasia, and invasive cancer"],"prefix":"10.1002","volume":"83","author":[{"given":"Paul K.S.","family":"Chan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih\u2010Jen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo 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