{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T09:01:52Z","timestamp":1772010112215,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,10,27]],"date-time":"2010-10-27T00:00:00Z","timestamp":1288137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HCV replication in extra\u2010hepatic reservoirs has been suggested to occur in many tissues including PBMCs. A recent study showed evidence for compartmentalization and evolution of HCV in PBMCs. However, the cells that support HCV replication in PBMCs have not been identified. In this study we have fractionated the PBMC from HIV\/HCV co\u2010infected patients into T, monocytes, B and NK cells, and most of the HCV was located in CD3\u2010cell fractions. Protease treatment of PBMCs to remove cell surface receptors resulted in the loss of HCV RNA suggesting that most of the HCV is present on the cell surface. PBMCs were treated by freeze\u2013thaw nuclease method that would protect the HCV RNA in the virus but not the intracellular viral RNA. Data from this analysis support the conclusion that most of HCV is present on the cell surface. Even though the presence of minus strand RNA in PBMCs suggests that a low level HCV replication takes place within the PBMCs of HIV\/HCV co\u2010infected individuals, HCV in PBMC is present mainly on the surface of non\u2010T cells, mostly on NK, monocytes and B cells. These results suggest a unique pathogenic role of NK, monocyte and B cells as carriers of HCV. J. Med. Virol. 82:2032\u20132037, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21906","type":"journal-article","created":{"date-parts":[[2010,10,27]],"date-time":"2010-10-27T18:20:53Z","timestamp":1288203653000},"page":"2032-2037","source":"Crossref","is-referenced-by-count":20,"title":["HCV in peripheral blood mononuclear cells are predominantly carried on the surface of cells in HIV\/HCV co\u2010infected individuals"],"prefix":"10.1002","volume":"82","author":[{"given":"Ven","family":"Natarajan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shyam","family":"Kottilil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"Hazen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Adelsberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison A.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A","family":"Polis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A","family":"Kovacs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,10,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80381-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300409"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21283"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/518251"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/323391"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200410210-00008"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1997.4581355.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e31814a4e7d"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/509897"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.15.7024-7031.2000"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v7.i2.228"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"3986","DOI":"10.1182\/blood.V95.12.3986.012k39_3986_3989","article-title":"Detection of active hepatitis C virus and hepatitis G virus\/GB virus C replication in bone marrow in human subjects","volume":"95","author":"Radkowski M","year":"2000","journal-title":"Blood"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.8.5769-5773.1997"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.59.081906.081110"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.284.4.450"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2326-5-2"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21906","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T10:39:54Z","timestamp":1696847994000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,27]]},"references-count":16,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1002\/jmv.21906"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21906","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,27]]}}}