{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:47:53Z","timestamp":1761662873791},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,9,17]],"date-time":"2010-09-17T00:00:00Z","timestamp":1284681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti\u2010HCV antibodies using a third\u2010generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the \u201cBanco Nacional de Sangre de la Cruz Roja Colombiana,\u201d Bogot\u00e1, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180\u2009bp of the 5\u2032UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5\u2032UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380\u2009bp from the NS5B region; and a segment of 391\u2009bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV\u20101b was introduced into Bogot\u00e1 around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti\u2010HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogot\u00e1, as is the case in other South American countries. J. Med. Virol. 82:1889\u20131898, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21908","type":"journal-article","created":{"date-parts":[[2010,9,17]],"date-time":"2010-09-17T15:33:01Z","timestamp":1284737581000},"page":"1889-1898","source":"Crossref","is-referenced-by-count":22,"title":["Molecular characterization, distribution, and dynamics of hepatitis C virus genotypes in blood donors in Colombia"],"prefix":"10.1002","volume":"82","author":[{"given":"M\u00f3nica Viviana Alvarado","family":"Mora","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Malta","family":"Romano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele Soares","family":"Gomes\u2010Gouv\u00eaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Fernanda","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flair Jos\u00e9","family":"Carrilho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Renato Rebello","family":"Pinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199111073251901"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000800019"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000600016"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(05)80032-0"},{"key":"e_1_2_6_6_1","first-page":"12","article-title":"Serological markers for communicable diseases in blood donors","volume":"19","author":"Blood bank situation in Latin America","year":"1996","journal-title":"Epidemiol Bull"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.suppl_1.I1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.17.8234"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02463-05"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10210"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21112"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21607"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-7-214"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2003000600003"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7930138.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)90494-8"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2007000400013"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03412.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.3.175"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21276"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(99)00036-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2007.00796.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91446-H"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92775-W"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260431"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199708)52:4<391::AID-JMV8>3.0.CO;2-X"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510290448"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2005.00649.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10298"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2007.0144"},{"key":"e_1_2_6_32_1","first-page":"182","volume-title":"Genotypes and genetic variation of Hepatitis C virus In The molecular medicine of viral Hepatitis","author":"Maertens G","year":"1997"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh088"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761998000300004"},{"key":"e_1_2_6_35_1","first-page":"1035","article-title":"Prevalence and genotypes of hepatitis C virus in blood donors and in patients with chronic liver disease and hepatocarcinoma in a Chilean population","volume":"126","author":"Munoz G","year":"1998","journal-title":"Rev Med Chil"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1086\/422606"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-122-3-199502010-00001"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20468"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(03)00088-3"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.2004.00368.x"},{"key":"e_1_2_6_41_1","article-title":"VGDN consortium, 2010.Social networks shape the transmission dynamics of hepatitis C virus","author":"Romano CM","journal-title":"PLoS One"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(200002)60:2<122::AID-JMV3>3.0.CO;2-#"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-001-0018-9"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.63.242"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(03)00070-3"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.12-29.2005"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.924"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-74-11-2391"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-78-2-321"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)32092-7"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2516(98)80002-2"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-007-2030-3"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_2_6_54_1","first-page":"13","volume-title":"Consensus statement on screening of blood donations for infectious agents transmissible through blood transfusion","author":"WHO","year":"1991"},{"key":"e_1_2_6_55_1","unstructured":"ZwicklDJ.2006.Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion: The University of Texas at Austin."}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21908","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21908","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T15:10:55Z","timestamp":1697641855000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,17]]},"references-count":54,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1002\/jmv.21908"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21908","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,17]]}}}