{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:15:45Z","timestamp":1761765345497},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,10,27]],"date-time":"2010-10-27T00:00:00Z","timestamp":1288137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of NO\u2010metoprolol, that is, 3\u2010nitrooxypivaloyl metoprolol\u2010amide, a novel NO\u2010releasing derivative of the \u03b21\u2010blocking drug metoprolol was investigated in A.CA\/SnJ mice infected with coxsackievirus B3 (CVB3) and compared to metoprolol and placebo. Daily treatment of mice with the respective drug started immediately (experiment A) or 3 days after virus infection (experiment B) and was continued until day 13 post\u2010infection (p.i.). Two doses of NO\u2010metoprolol were administered. Body mass differences, viral load, and histopathological signs of myocarditis were compared between the several groups. As a result, NO\u2010metoprolol diminished significantly the body weight loss, the viral load and the histopathology, whereas metoprolol treatment led solely to a significant attenuation of myocardial damage. In experiment A, low dose NO\u2010metoprolol decreased significantly enteroviral copy numbers. Both doses of NO\u2010metoprolol had a significant effect on reduction of myocardial infiltrates and fibrosis. The data suggest that delayed drug administration might more advantageous. Both doses of NO\u2010metoprolol reduced significantly the scores of four tested parameters compared to placebo. Body weight loss, virus titers, plus\u2010strand as well as minus\u2010strand enteroviral RNA levels, infiltration and fibrosis scores were diminished significantly when NO\u2010metoprolol was given 3 days p.i. In addition, a significant difference regarding the enteroviral copy numbers was observed between low dose NO\u2010metoprolol\u2010 and metoprolol\u2010treated mice. Treatment with metoprolol reduced insignificantly the viral load and body weight loss (experiment A and B) but led to a significant reduction of myocardial histopathology in experiment A. The results indicate that NO\u2010metoprolol treatment has a greater therapeutic benefit than metoprolol. J. Med. Virol. 82:2043\u20132052, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21928","type":"journal-article","created":{"date-parts":[[2010,10,27]],"date-time":"2010-10-27T18:20:53Z","timestamp":1288203653000},"page":"2043-2052","source":"Crossref","is-referenced-by-count":5,"title":["Cardioprotective effect of NO\u2010metoprolol in murine coxsackievirus B3\u2010induced 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