{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:24:33Z","timestamp":1791339873026,"version":"4.3.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T00:00:00Z","timestamp":1305158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A high human T\u2010cell lymphotropic virus type 1 (HTLV\u20101) proviral load is described in HTLV\u20101\u2010associated diseases, especially HAM\/TSP. However, the cut\u2010off value to define high levels of HTLV\u20101 proviral load is not well established. 281 HTLV\u20101\u2010infected patients from the HTLV reference center in Salvador, Brazil, were followed from 2005 to 2008. Patients were classified as asymptomatic, possible\u2010, probable\u2010, and definite\u2010HAM\/TSP, in accordance with diagnostic criteria proposed by De Castro\u2010Costa et al. (2006): AIDS Res Hum Retroviruses 22:931\u2013935. HTLV\u20101 proviral load was determined using real\u2010time PCR. A receiver operator characteristic (ROC) curve was constructed using only asymptomatic individuals and definite\u2010HAM\/TSP patients. The ROC curve was used to predict the proviral load level that differentiates these two groups. Out of 281 patients, 189 were asymptomatic and 92 were diagnosed with HAM\/TSP (22 possible, 23 probable, 47 definite). The mean HTLV\u20101 proviral load was higher in possible\u2010 (89,104\u2009\u00b1\u200993,006 copies\/10<jats:sup>6<\/jats:sup> PBMC), \u2010probable (175,854\u2009\u00b1\u2009128,083 copies\/10<jats:sup>6<\/jats:sup> PBMC), and definite\u2010HAM\/TSP patients (150,667\u2009\u00b1\u2009122,320 copies\/10<jats:sup>6<\/jats:sup> PBMC), when compared to asymptomatic individuals (27,178\u2009\u00b1\u200941,155 copies\/10<jats:sup>6<\/jats:sup> PBMC) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). A comparison of all HAM\/TSP groups showed the highest proviral loads in probable\u2010HAM\/TSP patients, yet the differences in mean values were not statistically significant. The ROC curve suggested a value of 49,865 copies\/10<jats:sup>6<\/jats:sup> PBMC, with 87% sensitivity (95% CI\u2009=\u200974\u201395) and 81% specificity (95% CI\u2009=\u200975\u201386), as the best proviral load cut\u2010off point to differentiate definite HAM\/TSP patients from asymptomatic individuals. HTLV\u20101 proviral loads are higher in groups of infected patients with neurological symptoms and may represent a relevant biological marker of disease progression. J. Med. Virol. 83:1269\u20131274, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.22087","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T20:43:43Z","timestamp":1305233023000},"page":"1269-1274","source":"Crossref","is-referenced-by-count":68,"title":["Human T cell lymphotropic virus type 1 (HTLV\u20101) proviral load of HTLV\u2010associated myelopathy\/tropical spastic paraparesis (HAM\/TSP) patients according to new diagnostic criteria of HAM\/TSP"],"prefix":"10.1002","volume":"83","author":[{"given":"Maria Fernanda Rios","family":"Grassi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana Nilla","family":"Olavarria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon de Almeida","family":"Kruschewsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita 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