{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T12:15:54Z","timestamp":1721477754081},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,7,7]],"date-time":"2011-07-07T00:00:00Z","timestamp":1309996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid detection of viral pathogens is crucial for antiviral therapy. High\u2010density 60\u201370\u2010mer oligonucleotide microarrays have been explored for broad detection of many viruses. However, relatively low specificity and the complex analytical processes are the major limitations when pan\u2010viral oligonucleotide microarrays are used to detect viral pathogens. In this study, genus\u2010specific oligonucleotides were used as probes and modified sample preparations were carried out to improve the specificity and accuracy of the pan\u2010viral oligonucleotide microarray. Genus\u2010specific 63\u2010mer oligonucleotide probes were used for screening human pathogenic RNA viruses. A total of 628 oligonucleotide probes covering 32 RNA viral genera from 14 viral families were used. The number of oligonucleotide probes was decreased to simplify the analytical process of hybridization and to minimize cross\u2010hybridization. Host genomes were removed by DNase I\/RNase T1 digestion before viral nucleic acid extraction, and non\u2010ribosomal hexanucleotides were used for reverse transcription to minimize interference of host genomes. Cultured viruses were used for microarray validation. The microarray was validated by cultured isolates that belonged to five viral genera. By using DNase I\/RNase T1 digestion before viral nucleic acid extraction and non\u2010ribosomal hexanucleotides for reverse transcription, the specificity of the microarray was improved. Furthermore, the analytical process of hybridization results was simplified. The specificity of pan\u2010viral microarray could be improved by using genus\u2010specific oligonucleotides as probes and by using non\u2010ribosomal hexanucleotides for reverse transcription. Combined with subsequent degenerate reverse transcriptase\u2010polymerase chain reaction and sequencing processes, this improved genus\u2010specific oligonucleotides microarray provides a relatively flexible strategy for diagnosis of RNA virus diseases. J. Med. Virol. 83:1624\u20131630, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.22157","type":"journal-article","created":{"date-parts":[[2011,7,7]],"date-time":"2011-07-07T17:52:33Z","timestamp":1310061153000},"page":"1624-1630","source":"Crossref","is-referenced-by-count":8,"title":["Improvement of the specificity of a pan\u2010viral microarray by using genus\u2010specific oligonucleotides and reduction of interference by host genomes"],"prefix":"10.1002","volume":"83","author":[{"given":"Xiaoping","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengfeng","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinhui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.211424698"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504666102"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.515"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5811-5818.2004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-232"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni064"},{"key":"e_1_2_7_8_1","volume-title":"Virus taxonomy. 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