{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:58:51Z","timestamp":1790233131100,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T00:00:00Z","timestamp":1320278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human adenoviruses species D (HAdV\u2010D) are known to cause severe epidemic keratoconjunctivitis. However, the isolation rate of HAdV\u2010D is not high, because HAdV\u2010D is usually slow to propagate. Although new types of HAdV\u2010D have been reported, accurate surveillance has not been performed because of difficulties in culturing the viruses and lack of a practical identification method. In this study, HAdV\u2010Ds were detected and identified from patients with epidemic keratoconjunctivitis in the Fukui Prefecture during 1995\u20132010 by PCR, loop\u2010mediated isothermal amplification (LAMP) of DNA, and conventional virus isolation and neutralization tests. All samples were subjected to culture and PCR and LAMP. A total of 124 strains of HAdV\u2010D were detected from 157 patients with epidemic keratoconjunctivitis. The strains consisted of the following types: D8 (n\u2009=\u20098), D19 (n\u2009=\u20094), D37 (n\u2009=\u200940), D53 (n\u2009=\u20095), D54 (n\u2009=\u200966), and D56 (n\u2009=\u20091). Among these, D53, D54, and D56 are new types that have been reported recently. The results of this study demonstrated that new types of HAdV\u2010D caused epidemic keratoconjunctivitis during 1995\u20132010, and included an outbreak of keratoconjunctivitis caused by HAdV\u2010D54. The LAMP method was able to detect and identify HAdV\u2010D53 and HAdV\u2013D54 in 1\u2009hr, and may therefore be applicable for use at the bedside. J. Med. Virol. 84:81\u201386, 2011. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.22252","type":"journal-article","created":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T10:49:35Z","timestamp":1320317375000},"page":"81-86","source":"Crossref","is-referenced-by-count":35,"title":["Surveillance of Adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 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