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The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture\u2010ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV\u2010infected material was assessed by an alternative method combining a single NS1\u2010directed monoclonal antibody and the SELDI\u2010TOF\/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno\u2010dominant peptide located on the C\u2010terminal part of the protein. The NS1 SELDI\u2010TOF assay is specific, has a sensitivity level close to capture\u2010ELISAs and is potentially useful for a coupled serotyping\/detection assay or for the detection of subtle post\u2010translational modifications on the protein. J. Med. 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