{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T11:37:09Z","timestamp":1717587429586},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T00:00:00Z","timestamp":1329264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The high incidence of progressive multifocal leukoencephalopathy (PML) among individuals with acquired immunodeficiency syndrome (AIDS) is similar to the incidence of other immunocompromised diseases. The pathogenic JC virus (JCV) with rearranged regulatory regions (PML\u2010type) causes PML, a demyelinating disease in the brains of immunocompromised patients. In a previous study, Tat protein, encoded by human immunodeficiency virus type 1 (HIV\u20101), markedly enhanced the expression of a reporter gene under control of the JCV late promoter. In order to examine the enhancement of JCV replication by Tat protein, the neuroblastoma cell line IMR\u201032 was used because it enables IMR\u201032\u2010adapted JCV. The extent of JCV replication in IMR\u201032 cells treated with Tat protein was significantly higher than that in untreated IMR\u201032 cells. The enhancement of JCV propagation by Tat protein was also examined using IMR\u201032\u2010derived JCV producing (JCI) cells which continuously produce JCV. Treatment of JCI cells with Tat protein led to a significant increase in the titers of progeny viruses. It has also been shown that Tat protein leads to a decrease in the expression of purine\u2010rich element binding protein \u03b1 (Pur\u03b1) as an important mediator of JCV replication in IMR\u201032 cells. Thus, it is probable that Tat protein enhances JCV replication in IMR\u201032 cells via the down\u2010regulation of Pur\u03b1 expression and cell proliferation. To our knowledge, this is the first report that exogenous Tat protein enhances the replication of JCV efficiently in neuroblastoma cell lines. J. Med. Virol. 84:555\u2013561, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23239","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T19:51:29Z","timestamp":1329335489000},"page":"555-561","source":"Crossref","is-referenced-by-count":9,"title":["Exogenous human immunodeficiency virus\u20101 protein, tat, enhances replication of JC virus efficiently in neuroblastoma cell lines"],"prefix":"10.1002","volume":"84","author":[{"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Kameoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Sugiura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nakamichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiyoko","family":"Nukuzuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Miyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Takegami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430104"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2003.50056"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"4150","DOI":"10.1128\/jvi.70.6.4150-4156.1996","article-title":"Evidence that replication of human neurotropic JC virus DNA in glial cells is regulated by the sequence\u2010specific single\u2010stranded DNA\u2010binding protein Pur\u03b1","volume":"70","author":"Chang CF","year":"1996","journal-title":"J Virol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.4.1087"},{"key":"e_1_2_6_6_1","first-page":"887","article-title":"GA\/GC\u2010rich sequence confers Tat responsiveness to human neurotropic virus promoter, JCL, in cells derived from central nervous system","volume":"8","author":"Chowdhury M","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0037"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-7-1543"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2004.23.858"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/13550280009015824"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3695"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(67)90307-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.003441"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.14112"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890470413"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2009.00156.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2009.00166.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00278.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.1.49"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.9.3479"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1659"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23239","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23239","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:55:39Z","timestamp":1700589339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,15]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/jmv.23239"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23239","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,15]]}}}