{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:31:23Z","timestamp":1790040683276,"version":"4.0.1"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T00:00:00Z","timestamp":1342569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zika virus (ZIKV) is a mosquito\u2010borne flavivirus. Infection results in a dengue\u2010like illness with fever, headache, malaise, and a maculopapular rash. Nearly all cases are mild and self\u2010limiting but in 2007, a large outbreak of ZIKV was reported from the island of Yap (in Micronesia, northwest of Indonesia). Singapore is already endemic for dengue, and its impact on public health and economic burden is significant. Other dengue\u2010like infections (e.g., Chikungunya virus) are present. Yet only 10% of reported dengue cases have laboratory confirmation. The identification and control of other dengue\u2010like, mosquito\u2010transmitted infections is thus important for the health of Singapore's population, as well as its economy. Given that ZIKV shares the same <jats:italic>Aedes<\/jats:italic> mosquito vector with both dengue and Chikungunya, it is possible that this virus is present in Singapore and causing some of the mild dengue\u2010like illness. A specific and sensitive one\u2010step, reverse transcription polymerase chain reaction (RT\u2010PCR) with an internal control (IC) was designed and tested on 88 archived samples of dengue\u2010negative, Chikungunya\u2010negative sera from patients presenting to our hospital with a dengue\u2010like illness, to determine the presence of ZIKV in Singapore. The assay was specific for detection of ZIKV and displayed a lower limit of detection (LoD) of 140 copies viral RNA\/reaction when tested on synthetic RNA standards prepared using pooled negative patient plasma. Of the 88 samples tested, none were positive for ZIKV RNA, however, the vast majority of these were from patients admitted to hospital and further study may be warranted in community\u2010based environments. J. Med. Virol. 84:1501\u20131505, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23241","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T19:52:20Z","timestamp":1343073140000},"page":"1501-1505","source":"Crossref","is-referenced-by-count":162,"title":["A diagnostic polymerase chain reaction assay for Zika virus"],"prefix":"10.1002","volume":"84","author":[{"given":"Michelle N.D.","family":"Balm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Kiat","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Kai","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn S.C.","family":"Koay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian W.","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(52)90042-4"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0805715"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400025997"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.05.005"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1705.101939"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1509.090442"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.4977-4983.2005"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1408.080287"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1605.091006"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(54)90006-1"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1969.18.411"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(82)90161-4"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2008.01208.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(81)90100-0"},{"key":"e_1_2_5_16_1","first-page":"585","article-title":"Immunological surveys of arbovirus infections in South\u2010East Asia, with special reference to dengue, chikungunya and Kyasanur Forest disease","volume":"44","author":"Rao TR","year":"1971","journal-title":"Bull World Health Organ"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/605496"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.10.008"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:31:40Z","timestamp":1700587900000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,18]]},"references-count":17,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1002\/jmv.23241"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23241","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,18]]}}}