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Aside from poliovirus, which is the target pathogen, isolation, and identification of non\u2010polio enteroviruses (NPEVs) is also done by neutralization test using pools of antisera which can only identify limited number of NPEVs. In the Philippines, despite the significant number of isolated NPEVs, no information is available with regard to its occurrence, diversity, and pattern of circulation. In this study, a total of 790 NPEVs isolated from stool samples submitted to the National Reference Laboratory from 1992 to 2008 were analyzed; neutralization test was able to type 55% (442) of the isolates. Of the remaining 356 isolates, which were untyped by using neutralization test, 348 isolates were analyzed further by RT\u2010PCR targeting the VP1 gene. A total of 47 serotypes of NPEV strains were identified using neutralization test and molecular typing, including 28 serotypes of human enterovirus B (HEV\u2010B), 12 serotypes of HEV\u2010A, and 7 of HEV\u2010C. The HEV\u2010B group (625\/790; 79%) constituted the largest proportion of isolates, followed by HEV\u2010C (108\/790; 13.7%), HEV\u2010A (57\/790; 7.2%), and no HEV\u2010D. Coxsackievirus (CV) B, echovirus (E)6, E11, and E13 were the most frequent isolates. E6, E11, E13, E14, E25, E30, E33, CVA20, and CVA24 were considered as endemic strains, some NPEVs recurred and few serotypes existed only for 1\u20133 years during the study period. Despite some limitations in this study, plural NPEVs with multiple patterns of circulation in the Philippines for 17 years were identified. J. Med. Virol. 84:624\u2013631, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23242","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T19:51:29Z","timestamp":1329335489000},"page":"624-631","source":"Crossref","is-referenced-by-count":45,"title":["Detection of non\u2010polio enteroviruses from 17 years of virological surveillance of acute flaccid paralysis in the Philippines"],"prefix":"10.1002","volume":"84","author":[{"given":"Lea Necitas","family":"Apostol","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Analisa","family":"Bautista","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel","family":"Galang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fem Julia","family":"Paladin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Fuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Socorro","family":"Lupisan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remigio","family":"Olveda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Oshitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.20.12650-12657.2005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.0810.584"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a116471"},{"key":"e_1_2_6_5_1","first-page":"245","article-title":"Isolation of a variant of enterovirus 70 from a patient during an epidemic of acute haemorrhagic conjunctivitis in Pune in 1991","volume":"38","author":"Bhide V","year":"1994","journal-title":"Acta Virol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21122"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.16.8973-8984.2003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890310313"},{"key":"e_1_2_6_9_1","first-page":"383","article-title":"Nonpolio enterovirus infection in the neonate and young infant","volume":"10","author":"Hawkes M","year":"2005","journal-title":"Paediatr Child Health"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909233411301"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.87.4.316"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800058982"},{"key":"e_1_2_6_13_1","unstructured":"Infectious Disease Surveillance Center.2000.Enterovirus Surveillance in Japan 1982\u20131999.Infectious Agents Surveillance Reports (IASR)."},{"key":"e_1_2_6_14_1","unstructured":"Infectious Disease Surveillance Center.2002.The trend of enterovirus isolation in association with aseptic meningitis 1999\u20132002.Infectious Agents Surveillance Reports (IASR). pp 193\u2013194."},{"key":"e_1_2_6_15_1","unstructured":"Infectious Disease Surveillance Center.2008.Infectious Agents Surveillance Report: Virus isolation\/detection from aseptic meningitis cases 2003\u20132008."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20533"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2008.06.008"},{"key":"e_1_2_6_18_1","first-page":"16","article-title":"Circulating vaccine\u2010derived polioviruses: Current state of knowledge","volume":"82","author":"Kew O","year":"2004","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/02724936.1998.11747935"},{"key":"e_1_2_6_20_1","first-page":"1","article-title":"Enterovirus surveillance\u2014United States, 1970\u20132005","volume":"55","author":"Khetsuriani N","year":"2006","journal-title":"MMWR Surveill Summ"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/101053950201400103"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.49.8.1314"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0502.990212"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1128\/CMR.11.1.202","article-title":"Molecular typing of enteroviruses: Current status and future requirements. 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