{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:59:00Z","timestamp":1778183940892,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T00:00:00Z","timestamp":1336953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis E virus (HEV) causes a common infection in developing countries. HEV infection occurs as outbreaks, as sporadic clinical cases and as large epidemics in endemic areas. The objective of this study was to determine the presence of HEV infection in patients with clinical suspicion of hepatitis A virus (HAV) infection, referred to the Instituto Nacional de Higiene \u201cRafael Rangel\u201d in Venezuela. Seventy\u2010four sera were tested for anti\u2010HAV and anti\u2010HEV IgM antibodies. HEV\u2010RNA was amplified from anti\u2010HEV IgM positive sera using nested reverse transcription polymerase chain reaction for ORF1 (RNA dependent RNA polymerase region) and the amplicons sequenced for phylogenetic analysis. The frequency of anti\u2010HEV IgM was 22\/74 (30%) in the samples tested. Dual infection with HAV and HEV was found in 31% (12\/39) of anti\u2010HAV IgM positive patients. Viremia was detected in 3\/22 (14%) of sera positive for anti\u2010HEV IgM. Two HEV strains were classified as genotype 1 and one as genotype 3, which were closely related to Yam 67 (north of India) and US1 isolates from the USA, respectively. These findings suggest that HEV is an important cause of acute viral hepatitis in Venezuela as a single infection or co\u2010infection with HAV, with high morbidity in children and young adults suggesting that this infection is endemic in Venezuela. J. Med. Virol. 84: 1025\u20131029, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23277","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T15:05:08Z","timestamp":1337007908000},"page":"1025-1029","source":"Crossref","is-referenced-by-count":31,"title":["Molecular characterization of hepatitis E virus in patients with acute hepatitis in Venezuela"],"prefix":"10.1002","volume":"84","author":[{"given":"Cristina Guti\u00e9rrez","family":"Garc\u00eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doneyla","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Caridad Montalvo","family":"Villalba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flor 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