{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:53:55Z","timestamp":1791078835797,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T00:00:00Z","timestamp":1336953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pulmonary involvement has been identified in human T\u2010lymphotropic virus type I (HTLV\u2010I) carriers and patients with HTLV\u2010I\u2010associated myelopathy\/tropical spastic paraparesis (HAM\/TSP). However, the relationship between HTLV\u2010I infection and lung disease is poorly understood. The occurrence of HTLV\u2010I\u2010specific immune responses in the lungs of patients infected with HTLV\u2010I with pulmonary involvement was investigated. The frequency of HTLV\u2010I\u2010specific CD8+ cells and the amount of HTLV\u2010I proviral DNA were determined in bronchoalveolar lavage fluid cells and peripheral blood mononuclear cells (PBMCs) from five patients with HAM\/TSP and one HTLV\u2010I carrier who had pulmonary involvement. HTLV\u2010I\u2010specific CD8+ cells were detected by flow cytometry using human leukocyte antigen\/antigen complex multimers. The analysis of bronchoalveolar lavage fluid revealed lymphocytosis in five of six patients. HTLV\u2010I provirus was detected in the bronchoalveolar lavage fluid cells of all patients, and the proviral load in these cells was comparable to that in PBMCs. The frequency of HTLV\u2010I\u2010specific CD8+ cells in the bronchoalveolar lavage fluid cells was 5.1 times higher than that in PBMCs. Immunohistochemically, clusters formed by HTLV\u2010I\u2010specific CD8+ cells were detected in lung tissue by in situ tetramer staining. No samples were available from patients infected with HTLV\u2010I without lung disorders. Whether accumulation of CD8+ cells is specific to patients with pulmonary involvement remains unclear. These results indicate that HTLV\u2010I\u2010specific CD8+ cells accumulate and HTLV\u2010I\u2010infected cells exist in the lungs of patients infected with HTLV\u2010I with pulmonary involvement. J. Med. Virol. 84: 1120\u20131127, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23307","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T15:05:08Z","timestamp":1337007908000},"page":"1120-1127","source":"Crossref","is-referenced-by-count":20,"title":["Human T\u2010lymphotropic virus type I (HTLV\u2010I)\u2010specific CD8+ cells accumulate in the lungs of patients infected with HTLV\u2010I with pulmonary 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