{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T07:41:32Z","timestamp":1725781292348},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T00:00:00Z","timestamp":1342569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HIV co\u2010infection significantly impacts the natural history of hepatitis C virus (HCV) by increasing plasma HCV viral load, accelerating liver disease progression, and reducing rates of HCV clearance. Cytokines play an important role in regulating hepatic inflammation and fibrogenesis during chronic HCV infection, yet the impact of HIV on cytokine expression is unknown. In this study, an HCV continuous infection cell culture system was modified to permit co\u2010infection with HIV to test the hypothesis that virus\u2010induced disregulation of immune\u2010response genes, particularly interferons and TGF\u2010\u03b2, may create a permissive environment for the initial establishment of HIV\/HCV co\u2010infection in the host. CCR5\u2010expressing Huh\u20107.5 hepatoma cells were transduced with human CD4 antigen to allow HIV infection in vitro. Co\u2010infection of CD4<jats:sup>+<\/jats:sup> Huh\u20107.5 cells with HIV and HCV or co\u2010culture of HIV\u2010infected CD4<jats:sup>+<\/jats:sup> Huh\u20107.5 cells and HCV\u2010infected Huh\u20107.5 cells increased the level of HCV RNA compared to HCV mono\u2010infection. Quantitative gene expression analysis revealed HIV\u2010induced up regulation of most tested IFN family genes when compared to HCV or co\u2010infection. HCV infection induced up regulation of many TGF family genes that were subsequently down\u2010regulated in the presence of HIV or HIV\/HCV. Interestingly, co\u2010infection resulted in down regulation of several IFN genes and significant up regulation of TGF\u2010\u03b2 genes leading to an overall enhancement of HCV replication. These data suggest that HIV infection may influence HCV replication in vitro by increasing levels of HCV RNA, possibly through the differential regulation of endogenous IFN and TGF family genes. J. Med. Virol. 84:1344\u20131352, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23315","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T19:52:20Z","timestamp":1343073140000},"page":"1344-1352","source":"Crossref","is-referenced-by-count":7,"title":["Human immunodeficiency virus enhances hepatitis C virus replication by differential regulation of IFN and TGF family 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