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The objective of this study was to determine whether the DNAs of HBoV1\u20104 and PARV4 persist in human tissues long after primary infection. Biopsies of tonsillar tissue, skin, and synovia were examined for HBoV1\u20104 DNA and PARV4 DNA by PCR. Serum samples from the tissue donors were assayed for HBoV1 and PARV4 IgG and IgM antibodies. To obtain species\u2010specific seroprevalences for HBoV1 and for HBoV2\/3 combined, the sera were analyzed after virus\u2010like particle (VLP) competition. While HBoV1 DNA was detected exclusively in the tonsillar tissues of 16\/438 individuals (3.7%), all of them \u22648 years of age. HBoV2\u20104 and PARV4 DNAs were absent from all tissue types. HBoV1 IgG seroprevalence was 94.9%. No subject had HBoV1 or PARV4 IgM, nor did they have PARV4 IgG. The results indicate that HBoV1 DNA occurred in a small proportion of tonsils of young children after recent primary HBoV1 infection, but did not persist long in the other tissue types studied, unlike parvovirus B19 DNA. The results obtained by the PARV4 assays are in line with previous results on PARV4 epidemiology. J. Med. Virol. 84: 1267\u20131273, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23335","type":"journal-article","created":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T02:28:29Z","timestamp":1340072909000},"page":"1267-1273","source":"Crossref","is-referenced-by-count":20,"title":["Occurrence of human bocaviruses and parvovirus 4 in solid 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