{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T15:19:16Z","timestamp":1773155956737,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T00:00:00Z","timestamp":1348012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The polyomavirus JC (JCPyV) is a ubiquitous virus in humans, causing progressive multifocal leukoencephalopathy, a fatal demyelinating disease. JCPyV propagates in the adult kidney and excretes its progeny in urine, from which its DNA can be recovered readily. JCPyV isolates worldwide can be classified into 14 subtypes or genotypes, each associated with a specific geographical region. The European genotypes EU\u2010a\u2010b\u2010c are spread throughout Europe and Mediterranean areas. The major African genotype Af2 is spread not only throughout Africa but also in West and South Asia. A minor African genotype (Af1) occurs in Central and West Africa. Partially overlapping domains in Asia were occupied by various genotypes (e.g., B1\u2010a, \u2010b, \u2010d, B2, CY, MY, and SC). To characterize the subtypes of JCPyV prevalent in Tunisia, the presence of the virus was investigated by real\u2010time PCR in urine samples from 98 renal transplant recipients. For subtype identification, a 610\u2009bp typing region of the JCPyV genome was amplified from each urine sample, and its DNA sequence was determined. In the patients studied, the major African subtype Af2 was the predominant (62.5%), followed by the European subtype EU (33.5%). Only one case clustering with the Asian genotype SC (4%) was identified. The presence of the European subtype with high prevalence in this population suggests that the epidemiological distribution of JCPyV virus sequences in North Africa is related partially to the epidemiological data in Europe. J. Med. Virol. 84:1818\u20131824, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23385","type":"journal-article","created":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T15:21:45Z","timestamp":1348068105000},"page":"1818-1824","source":"Crossref","is-referenced-by-count":4,"title":["Distribution of JC polyomavirus genotypes in Tunisian renal transplant recipients between January 2008 and January 2011"],"prefix":"10.1002","volume":"84","author":[{"given":"Hanen","family":"Boukoum","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imen","family":"Nahdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabil","family":"Abid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Foulongne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wissal","family":"Sahtout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorsaf","family":"Zallema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Habib","family":"Skiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabra","family":"Aloui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelatif","family":"Achour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Segondy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahjoub","family":"Aouni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,9,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.159-164.1996"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050108"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14542"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/0471221945.ch18","volume-title":"Human polyomaviruses\u2014Molecular and clinical perspectives","author":"Agostini HT","year":"2001"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00028-07"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31816a33d4"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-10-2669"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.12.006"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/jmv.22035","article-title":"Distribution of BK polyomavirus genotypes in Tunisian renal transplant recipients","volume":"83","author":"Boukoum H","journal-title":"J Med Virol"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/13550280260422758"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761998000500010"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1128\/iai.33.1.319-321.1981","article-title":"Serological investigation of the possibility of congenital transmission of papovavirus JC","volume":"33","author":"Daniel R","year":"1981","journal-title":"Infect Immun"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/317927"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152586"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.37.5.578"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030064"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-5-919"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-10-2499"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.09.027"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170023"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-10-2491"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050125"},{"key":"e_1_2_5_26_1","first-page":"471","article-title":"A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences","volume":"47","author":"Kimura M","year":"1980","journal-title":"J Mol Evol"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.6.1128"},{"key":"e_1_2_5_28_1","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1128\/jcm.32.10.2359-2363.1994","article-title":"Transmission of the human polyomavirus JC virus occurs both within the family and outside the family","volume":"32","author":"Kitamura T","year":"1994","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_29_1","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1128\/jcm.35.5.1255-1257.1997","article-title":"Persistent JC virus (JCV) infection is demonstrated by continuous shedding of the same JCV strains","volume":"35","author":"Kitamura T","year":"1997","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/0471221945.ch19","volume-title":"Human polyomaviruses\u2014Molecular and clinical perspectives","author":"Knowles WA","year":"2001"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.04.008"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.6.1448-1451.1995"},{"key":"e_1_2_5_33_1","first-page":"2175","volume-title":"Fields virology","author":"Major EO","year":"2001"},{"key":"e_1_2_5_34_1","first-page":"12","article-title":"Human polyomaviruses in skin diseases","volume":"2011","author":"Moens U","year":"2011","journal-title":"Patholog Res Int"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/127.4.467"},{"key":"e_1_2_5_36_1","first-page":"1","article-title":"New human papovaviruses","volume":"22","author":"Padgett BL","year":"1976","journal-title":"Prog Med Virol"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91777-6"},{"key":"e_1_2_5_38_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1080\/13550280390241269","article-title":"Molecular analysis of JC virus genotypes circulating among the Italian healthy population","volume":"9","author":"Pagani E","year":"2003","journal-title":"J Neurovirol"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-002-2425-y"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80650-0"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s705-002-8299-4"},{"key":"e_1_2_5_42_1","first-page":"1364","article-title":"Human polyomavirus related to African green monkey lymphotropic polyomavirus","volume":"17","author":"Sauvage V","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.05.006"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02602-10"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.9191"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-001-0009-x"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.20208"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001024"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1159\/000150537"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4357-4361.2001"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1353\/hub.2002.0037"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.428"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-003-0214-6"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23385","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T05:53:08Z","timestamp":1700545988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,19]]},"references-count":53,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/jmv.23385"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23385","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,19]]}}}