{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:31:57Z","timestamp":1764603117745},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,10,10]],"date-time":"2012-10-10T00:00:00Z","timestamp":1349827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was designed to compare the sensitivity of a Sabin vaccine strain\u2010specific PCR assay and an enzyme\u2010linked immunosorbent assay with polyclonal cross\u2010absorbed antisera (PAb\u2010E) for intratypic differentiation (ITD) of polioviruses (PVs). These were used for the definitive characterization of the strains. Poliovirus strains isolated in L20B and RD cell lines were subjected to both PCR and ELISA. Both PCR and ELISA identified 3 (13.6%) out of 22 isolates, respectively as poliovirus Sabin 1. PCR identified 4 (18.2%) out of 22 isolates as poliovirus Sabin 2 and ELISA identified 2 (9.1%) out of 22 isolates as poliovirus Sabin 2. None of the two assay identified poliovirus Sabin 3. Both PCR and ELISA identified 12 (54.5%) out of 22 isolates, respectively as wild poliovirus (WPV) 1. None of the assays identified any of the isolates as WPV 2 and 3. Only PCR assay was able to identify the mixture of two poliovirus Sabin serotypes (a mixture of Sabin 1 and 2) and two mixtures of poliovirus Sabin 2 and 3. In this study, only ELISA was able to identified two invalid results. Invalid results observed in this study are of important practical implication to the emergence of vaccine\u2010derived poliovirus. This may have epidemic potential. Hence, the two ITD assays are of paramount importance for identification of PVs. It is therefore recommended in line with WHO guideline that at least two methods be used for the ITD of poliovirus isolates, and each method should be based on a different principle (i.e., antigenic and genetic properties). J. Med. Virol. 84:1975\u20131979, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23408","type":"journal-article","created":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T01:12:59Z","timestamp":1350609179000},"page":"1975-1979","source":"Crossref","is-referenced-by-count":3,"title":["Comparative study of molecular and antigenic characterization for intratypic differentiation (ITD) of poliovirus strains"],"prefix":"10.1002","volume":"84","author":[{"given":"A.O.","family":"Adedeji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.O.","family":"Okonko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.D.","family":"Adu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB2003.000-1092"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.03.009"},{"key":"e_1_2_6_4_1","first-page":"40","article-title":"Risk communication: The over\u2010looked factor in the Nigeria polio immunization boycott crisis","volume":"51","author":"Agbeyebe L","year":"2007","journal-title":"Niger Med Practitioner"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23184"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90434-D"},{"key":"e_1_2_6_7_1","first-page":"874","article-title":"Acute flaccid paralysis associated with circulating vaccine\u2010derived poliovirus\u2014Philippines","volume":"50","author":"Centers for Disease Control and Prevention (CDC)","year":"2001","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_6_8_1","first-page":"958","article-title":"Laboratory surveillance for wild and vaccine\u2010derived polioviruses, January 2004\u2013June 2005","volume":"54","author":"Centre for Disease Control and Prevention (CDC)","year":"2005","journal-title":"Morb Mortal Wkly Rep (MMWR)"},{"key":"e_1_2_6_9_1","first-page":"958","article-title":"Progress towards poliomyelitis eradication\u2010Nigeria, January 2004\u2013July 2005","volume":"54","author":"Centre for Disease Control and Prevention (CDC)","year":"2005","journal-title":"Morb Mortal Wkly Rep (MMWR)"},{"key":"e_1_2_6_10_1","first-page":"153","article-title":"Enterovirus surveillance\u2013United States, 2002\u20132004","volume":"55","author":"Centers for Disease Control and Prevention (CDC)","year":"2006","journal-title":"Morb Mortal Wkly Rep (MMWR)"},{"key":"e_1_2_6_11_1","first-page":"458","article-title":"Progress towards interruption of wild poliovirus transmission\u2010worldwide, January 2005\u2013March 2006","volume":"55","author":"Centre for Disease Control and Prevention (CDC)","year":"2006","journal-title":"Morb Mortal Wkly Rep (MMWR)"},{"key":"e_1_2_6_12_1","first-page":"278","volume":"56","author":"Centre for Disease Control and Prevention (CDC)","year":"2007","journal-title":"Morb Mortal Wkly Rep (MMWR)"},{"key":"e_1_2_6_13_1","first-page":"308","article-title":"Progress toward interruption of wild poliovirus transmission\u2014worldwide, 2008","volume":"58","author":"Centre for Disease Control and Prevention (CDC)","year":"2009","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_6_14_1","first-page":"1002","article-title":"Update on vaccine\u2010derived polioviruses\u2010worldwide, January 2008\u2013June 2009","volume":"58","author":"Centre for Disease Control and Prevention (CDC)","year":"2009","journal-title":"MMWR"},{"key":"e_1_2_6_15_1","first-page":"441","article-title":"Tracking progress toward global polio eradication\u2010worldwide, 2009\u20132010","volume":"60","author":"Centre for Disease Control and Prevention (CDC)","year":"2011","journal-title":"MMWR"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","unstructured":"DeL NottayB YangCF HollowayBP PallanschM KewOM.1995. 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