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The assay could discriminate SFTSV infection from other related viral diseases in human with a minimum detection limit of 10 viral RNA copies\/\u00b5l and was 1,000 times more sensitive than the conventional PCR. Strong linear correlations (r<jats:sup>2<\/jats:sup>\u2009&gt;\u20090.99) between the C<jats:sub>t<\/jats:sub> values and viral RNA standards over a linear range were obtained. The coefficients of variation of intra\u2010 and inter\u2010assay reproducibility were both less than 2%. The RT\u2010PCR was also shown to be highly specific, as no positive signals were detected for other related viruses. Evaluation of this assay with serum samples from laboratory confirmed cases and healthy donors showed 100% clinical diagnostic sensitivity and over 99% specificity. Clinical application with samples from 287 patients admitted to the hospital with suspected SFTSV infection showed that 15% were infected by SFTSV. This assay was rapid, requiring just over 2\u2009hr, including the nucleic acid extraction step. J. Med. Virol. 85:370\u2013377, 2013.  \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23415","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T20:56:44Z","timestamp":1354654604000},"page":"370-377","source":"Crossref","is-referenced-by-count":41,"title":["Development and application of a one\u2010step real\u2010time RT\u2010PCR using a minor\u2010groove\u2010binding probe for the detection of a novel bunyavirus in clinical 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