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In the present study, a novel functional T\u2010cell assay was developed to test the correlation between age and VZV\u2010specific T\u2010cell responses in peripheral blood from healthy individuals. Secondly, VZV\u2010specific T\u2010cell responses from renal transplant recipients were compared with healthy individuals. Monocytes were differentiated into mature monocyte\u2010derived dendritic cells (moDCs) and were infected with VZV. T\u2010cells were co\u2010cultured with autologous moDCs infected with VZV and subjected to flowcytometric analysis to identify the phenotype (i.e., na\u00efve [NA: CCR7<jats:sup>+<\/jats:sup>CD45RO<jats:sup>\u2212<\/jats:sup>], central [CM: CCR7<jats:sup>+<\/jats:sup>CD45RO<jats:sup>+<\/jats:sup>] and effector memory [EM: CCR7<jats:sup>\u2212<\/jats:sup>CD45RO<jats:sup>+<\/jats:sup>] T\u2010cells) and the frequency of VZV\u2010reactive T\u2010cell subsets by intra\u2010cellular IFN\u2010\u03b3 flowcytometry. In contrast to NA and CM T\u2010cells, the frequency of VZV\u2010reactive CD4 and CD8 EM T\u2010cells was inversely correlated with age (<jats:italic>P<\/jats:italic>\u2009=\u20090.0007 and <jats:italic>P<\/jats:italic>\u2009=\u20090.01). No difference was found in the percentage of VZV\u2010reactive CD4 NA, CM and EM T\u2010cells between transplant recipients and controls. However, the percentage of VZV\u2010reactive CD8 EM T\u2010cells was significantly lower in transplant recipients compared to controls (<jats:italic>P<\/jats:italic>\u2009=\u20090.02). In conclusion, moDCs infected with VZV are efficient antigen presenting cells applicable to enumerate and characterize the phenotype and differentiation status of the systemic VZV\u2010specific T\u2010cell response ex\u2010vivo. The data suggest that VZV\u2010reactive EM T\u2010cells are impaired in the elderly and renal transplant recipients. J. Med. Virol. 84:2018\u20132025, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23427","type":"journal-article","created":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T01:12:59Z","timestamp":1350609179000},"page":"2018-2025","source":"Crossref","is-referenced-by-count":29,"title":["Systemic varicella zoster virus reactive effector memory T\u2010cells impaired in the elderly and in kidney transplant recipients"],"prefix":"10.1002","volume":"84","author":[{"given":"Nicole M.","family":"van Besouw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges M.G.M.","family":"Verjans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joke M.","family":"Zuijderwijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolle 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