{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T22:21:35Z","timestamp":1775600495688,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Testicular germ cell tumors account for about 1% of all cancers. The incidence of these tumors is increasing and they represent the most common solid malignancies of young men aged 15\u201340 years with seminoma being one of the most common histotype. Pathogenesis of testicular germ cell tumors remains unknown and, although cryptorchidism is considered the main risk factor, there is evidence of an association with environmental and genetic risk factors. Human papillomaviruses (HPV) are a family of DNA viruses and represent a major risk factor for cervical cancer. In addition, they have been associated with other human non\u2010malignant and malignant diseases, including breast and head and neck cancer. HPV sequences have been detected throughout the male lower genitourinary tract as well as in seminal fluid and an increased testicular tumorigenesis has been reported in HPV transgenic mice. Aim of this study was to evaluate the potential involvement of HPV in human testicular tumorigenesis. Real\u2010time PCR employing GP5+\/GP6+ consensus HPV primers was used to examine the presence of HPV sequences in a subset of human seminoma (n\u2009=\u200961) and normal testicles (n\u2009=\u200923). None of the specimens tested displayed the presence of HPV DNA. These findings do not support an association between HPV and human seminoma and warrant further studies to assess definitively the role of these viruses in human testicular tumorigenesis. J. Med. Virol. 85:105\u2013109, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23431","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T17:41:12Z","timestamp":1350409272000},"page":"105-109","source":"Crossref","is-referenced-by-count":14,"title":["Lack of evidence for an association between seminoma and human papillomavirus infection using GP5+\/GP6+ consensus primers"],"prefix":"10.1002","volume":"85","author":[{"given":"Giorgia","family":"Bertazzoni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sgambato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Migaldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Grottola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria Teresa","family":"Sabbatini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Nanni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Farinetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Iachetta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabetta","family":"Giacobazzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Pecorari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca Reggiani","family":"Bonetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)42394-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-10-0031"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2009.06.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.03.020"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-4-1057"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/507432"},{"key":"e_1_2_6_8_1","volume-title":"Pathology and genetics of tumours of the urinary system and male genital organs","author":"Eble JN","year":"2004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-269"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600194"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130401500402"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-12-30"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2008.00983.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1128\/jcm.33.4.901-905.1995","article-title":"Group\u2010specific differentiation between high and low\u2010risk human papillomavirus genotypes by general primer\u2010mediated PCR and two cocktails of oligonucleotide probes","volume":"33","author":"Jacobs MV","year":"1995","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"3335","DOI":"10.1128\/jvi.65.6.3335-3339.1991","article-title":"Very high incidence of germ cell tumorigenesis (seminomagenesis) in human papillomavirus type 16 transgenic mice","volume":"65","author":"Kondoh G","year":"1991","journal-title":"J Virol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1016\/S0022-5347(17)32341-8","article-title":"Establishment and further characterization of a line of transgenic mice showing testicular tumorigenesis at 100% incidence","volume":"152","author":"Kondoh G","year":"1994","journal-title":"J Urol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.5858\/2005-129-1132-POHPDI","article-title":"Presence of human papillomavirus DNA in testicular biopsies from nonobstructive azoospermic men","volume":"129","author":"Martorell M","year":"2005","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70017-6"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910410314"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3003908"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1086\/340421"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.04.004"},{"key":"e_1_2_6_23_1","first-page":"3647","article-title":"Glutathione S\u2010transferase (GST) polymorphisms as risk factors for cancer in a highly homogeneous population from Southern Italy","volume":"22","author":"Sgambato A","year":"2002","journal-title":"Anticancer Res"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/IGC.0b013e31823c712e"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3176-3184.2004"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1086\/344354"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2010.196"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01713041"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23431","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23431","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:44:41Z","timestamp":1700657081000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,16]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/jmv.23431"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23431","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,16]]}}}