{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T20:45:09Z","timestamp":1771620309607,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The incidence of progressive multifocal leukoencephalopathy (PML) has increased due to the AIDS pandemic, hematological malignancies, and immunosuppressive therapies. Recently, the number of cases of monoclonal antibody\u2010associated PML has increased in patients treated with immunomodulatory drugs such as natalizumab. However, no common consensus regarding PML therapy has been reached in clinical studies. In order to examine the suppression of JC virus (JCV) replication by 3\u2010aminobenzamide (3\u2010AB), a representative PARP\u20101 inhibitor, a DNA replication assay was carried out using the neuroblastoma cell line IMR\u201032 and IMR\u2010adapted JCV. The suppression of JCV propagation by 3\u2010AB was also examined using JCI cells, which are a carrier culture producing continuously high JCV titers. The results indicated that PARP\u20101 inhibitors, such as 3\u2010aminobenzamide (3\u2010AB), suppress JCV replication and propagation significantly in vitro, as judged by DNA replication assay, hemagglutination, and real\u2010time PCR analysis. It has been also shown that 3\u2010AB reduced PARP\u20101 activity in IMR\u201032 cells. According to the results of the MTT assay, the enzyme activity of 3\u2010AB\u2010treated cells was slightly lower than that of DMSO\u2010treated cells. However, the significant suppression of JCV propagation is not related to the slight decrease in cell growth. To our knowledge, this is the first report that PARP\u20101 inhibitor suppresses the replication of JCV significantly in neuroblastoma cell lines via the reduction of PARP\u20101 activity. Thus, PARP\u20101 inhibitors also may be a novel therapeutic drug for PML. J. Med. Virol. 85:132\u2013137, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23443","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T17:41:23Z","timestamp":1350409283000},"page":"132-137","source":"Crossref","is-referenced-by-count":18,"title":["Suppressive effect of PARP\u20101 inhibitor on JC virus replication in vitro"],"prefix":"10.1002","volume":"85","author":[{"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Kameoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Sugiura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nakamichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiyoko","family":"Nukuzuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Takegami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1040837"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01614-08"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70161-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/13550280802235916"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1040836"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103492"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.7.4273-4282.2003"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/313598"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199805073381903"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(67)90307-5"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70032-6"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.16.8931-8934.2004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05897-11"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.1.49"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200209060-00012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890470413"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2009.00156.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23239"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00406-08"},{"key":"e_1_2_6_21_1","first-page":"2110","article-title":"Definition of a continous human cell line derived from neruoblastoma","volume":"30","author":"Tumiowicz JJ","year":"1970","journal-title":"Cancer Res"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1659"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23443","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:44:19Z","timestamp":1700657059000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,16]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/jmv.23443"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23443","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,16]]}}}