{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:28:45Z","timestamp":1788323325698,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,14]],"date-time":"2012-11-14T00:00:00Z","timestamp":1352851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Herpes simplex virus (HSV) infections have become a public health problem worldwide. The emergence of acyclovir\u2010resistant viral strains and the failure of vaccination to prevent herpetic infections have prompted the search for new antiviral drugs. Accordingly, the present study was undertaken to synthesize chemically and evaluate Dermaseptin S\n                    <jats:sub>4<\/jats:sub>\n                    (S\n                    <jats:sub>4<\/jats:sub>\n                    ), an anti\u2010microbial peptide derived from amphibian skin, and its derivatives in terms of anti\u2010herpetic activity. The effects of biochemical modifications on their antimicrobial potential were also investigated. The peptides were incubated together with HSV\u20102 on target cells under various conditions, and the antiviral effects were examined via a cell metabolic labeling method. The findings revealed that DS\n                    <jats:sub>4<\/jats:sub>\n                    derivatives elicited concentration\u2010dependent antiviral activity at micromole concentrations. The biochemical modifications of S\n                    <jats:sub>4<\/jats:sub>\n                    allowed for the reduction of peptide cytotoxicity without altering antiviral activity. Dermaseptins were added at different times during the viral cycle to investigate the mode of antiviral action. At the highest non\u2010cytotoxic concentrations, most of the tested derivatives were noted to exhibit high antiviral activity particularly when pre\u2010incubated with free herpes viruses prior to infection. Among these peptides, K\n                    <jats:sub>4<\/jats:sub>\n                    K\n                    <jats:sub>20<\/jats:sub>\n                    S\n                    <jats:sub>4<\/jats:sub>\n                    exhibited the highest antiviral activity against HSV\u20102 sensitive and resistant strains. Interestingly, the antiviral activity of K\n                    <jats:sub>4<\/jats:sub>\n                    K\n                    <jats:sub>20<\/jats:sub>\n                    S\n                    <jats:sub>4<\/jats:sub>\n                    was effective on both acyclovir\u2010resistant and \u2010sensitive viruses. The findings indicate that K\n                    <jats:sub>4<\/jats:sub>\n                    K\n                    <jats:sub>20<\/jats:sub>\n                    S\n                    <jats:sub>4<\/jats:sub>\n                    can be considered a promising candidate for future application as a therapeutic virucidal agent for the treatment of herpes viruses. J. Med. Virol. 85:272\u2013281, 2013.  \u00a9 2012 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jmv.23450","type":"journal-article","created":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T11:49:01Z","timestamp":1352980141000},"page":"272-281","source":"Crossref","is-referenced-by-count":45,"title":["In vitro antiviral activity of dermaseptin S\n                    <sub>4<\/sub>\n                    and derivatives from amphibian skin against herpes simplex virus type 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