{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:29:37Z","timestamp":1784950177112,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) assay of the upper respiratory tract is used increasingly to diagnose lower respiratory tract infections. The cycle threshold (C<jats:sub>T<\/jats:sub>) values of qRT\u2010PCR are continuous, semi\u2010quantitative measurements of viral load, although interpretation of diagnostic qRT\u2010PCR results are often categorized as positive, indeterminate, or negative, obscuring potentially useful clinical interpretation of C<jats:sub>T<\/jats:sub> values. From 2008 to 2010, naso\/oropharyngeal swabs were collected from outpatients with influenza\u2010like illness, inpatients with severe respiratory illness, and asymptomatic controls in rural Kenya. C<jats:sub>T<\/jats:sub> values of positive specimens (i.e., C<jats:sub>T<\/jats:sub> values\u2009&lt;\u200940.0) were compared by clinical severity category for five viruses using Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010test and logistic regression. Among children &lt;5 years old we tested with respiratory syncytial virus (RSV), inpatients had lower median C<jats:sub>T<\/jats:sub> values (27.2) than controls (35.8, <jats:italic>P<\/jats:italic>\u2009=\u20090.008) and outpatients (34.7, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Among children and older patients infected with influenza virus, outpatients had the lowest median C<jats:sub>T<\/jats:sub> values (29.8 and 24.1, respectively) compared with controls (<jats:italic>P<\/jats:italic>\u2009=\u20090.193 for children, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 for older participants) and inpatients (<jats:italic>P<\/jats:italic>\u2009=\u20090.009 for children, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 for older participants). All differences remained significant in logistic regression when controlling for age, days since onset, and coinfection. C<jats:sub>T<\/jats:sub> values were similar for adenovirus, human metapneumovirus, and parainfluenza virus in all severity groups. In conclusion, the C<jats:sub>T<\/jats:sub> values from the qRT\u2010PCR of upper respiratory tract specimens were associated with clinical severity for some respiratory viruses. J. Med. Virol. 85:924\u2013932, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23455","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T14:56:51Z","timestamp":1363618611000},"page":"924-932","source":"Crossref","is-referenced-by-count":83,"title":["Association of the C<sub>T<\/sub> values of real\u2010time PCR of viral upper respiratory tract infection with clinical severity, Kenya"],"prefix":"10.1002","volume":"85","author":[{"given":"James A.","family":"Fuller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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