{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:49:12Z","timestamp":1764402552962},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T00:00:00Z","timestamp":1353369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Occult HCV infection is a form of chronic HCV infection characterized by absence of detectable anti\u2010HCV antibodies or plasma HCV\u2010RNA but presence of HCV\u2010RNA in liver biopsy and\/or peripheral blood mononuclear cells (PBMCs). The aim of this study was to determine the presence of HCV\u2010RNA in PBMCs of patients with lymphoproliferative disorders. One hundred and four consecutive patients with lymphoproliferative disorders admitted to Firouzgar Hospital from January 2010 to March 2011 were recruited in this cross\u2010sectional study. A 6\u2010ml sample of whole blood was taken from the patients, the total RNA was extracted from the samples after the separation of plasma and PBMCs. The HCV\u2010RNA of the samples was amplified by reverse transcriptase\u2010nested polymerase chain reaction (RT\u2010nested PCR). The HCV genotypes of the positive samples were tested using the INNO\u2010LiPA\u2122 HCV II kit, and the HCV genotypes were then confirmed by sequencing of the 5\u2032\u2010UTR fragments after the PCR products were cloned into a pJET1.2\/blunt cloning vector. The mean age of the patients was 48.3\u2009\u00b1\u20091.76 years (range: 16\u201383). HCV\u2010RNA was found in PBMCs from 2 (1.9%) of the 104 patients. Genotyping showed that the patients were infected with HCV subtype 1a. One patient suffered non\u2010Hodgkin's lymphoma and the other suffered chronic lymphocytic leukemia. Patients with lymphoproliferative disorders with negative anti\u2010HCV antibodies and negative plasma HCV\u2010RNA may have occult HCV infection. Therefore, in the absence of a liver biopsy, the testing of PBMCs for the detection of genomic HCV\u2010RNA may be beneficial. J. Med. Virol. 85:235\u2013240, 2013.  \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23460","type":"journal-article","created":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T20:48:59Z","timestamp":1353444539000},"page":"235-240","source":"Crossref","is-referenced-by-count":30,"title":["Prevalence of occult hepatitis C virus infection in Iranian patients with lymphoproliferative 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