{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T18:49:55Z","timestamp":1773082195691,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>On December 29, 2011, a man infected with a subclade of the H5N1 virus was confirmed in Shenzhen, China. The clinical symptoms and immune factors of the patient were investigated and the phylogenetic and molecular characteristics of the virus were analyzed. High fever, rapid development of serious pneumonia and multi\u2010organ failure were the main clinical symptoms. Arterial blood gas analysis showed that PaCO<jats:sub>2<\/jats:sub> rose sharply and PO<jats:sub>2<\/jats:sub> decreased. Leukocyte and platelet counts decreased rapidly. Peripheral blood lymphocyte counts indicated lymphopenia and inverted ratios of CD4<jats:sup>+<\/jats:sup> to CD8<jats:sup>+<\/jats:sup> cells. Cytokine analysis showed that the levels of serum IL\u20106, IL\u201010, and IFN\u2010r continued to increase, whereas the levels of IL\u201012 and TNFs decreased during the clinical course. MCP\u20101 and IP\u201010 remained at a high level after infection. Phylogenetic analysis confirmed that the virus A\/Shenzhen\/1\/2011 belongs to the new subclade 2.3.2.1. An Arg (R) insertion at P6 and an RP8I substitution in the HA cleavage site motif were detected in the virus. Compared to the vaccine strain, 16 specific substitutions occurred in the HA1 protein. Some of them were located on the receptor\u2010binding site, glycosylation site and the region of the antigenic determinant. In summary, serious complications and immune system disorders were the main features of the infection with H5N1. Gene variation did not weaken the highly pathogenic features of viruses and the pathogenicity and antigenicity of the new subclade virus were changed. J. Med. Virol. 85:760\u2013768, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23492","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T14:56:51Z","timestamp":1363618611000},"page":"760-768","source":"Crossref","is-referenced-by-count":12,"title":["Clinical symptoms, immune factors, and molecular characteristics of an adult male in Shenzhen, China infected with influenza virus H5N1"],"prefix":"10.1002","volume":"85","author":[{"given":"Chunli","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shisong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanwu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinquan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511120103"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00592-10"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11772-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1477"},{"key":"e_1_2_6_6_1","unstructured":"Food and Agriculture Organization.2011a. Approaches to controlling preventing and eliminating H5N1 highly pathogenic Avian Influenza in endemic countries. Available:http:\/\/www.fao.org\/docrep\/014\/i2150e\/i2150e.pdf"},{"key":"e_1_2_6_7_1","unstructured":"Food and Agriculture Organization.2011b. Bird Flu rears its head again. Increased preparedness and surveillance urged against variant strain. Available:http:\/\/www.fao.org\/news\/story\/en\/item\/87196\/icode\/"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507415102"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00689-10"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/483535a"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-007-0611-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1703.100948"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2011.01297.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.07.003"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1702.100732"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1121586"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15595-5"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.07.007"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.037572-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06531"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.11.030"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.531"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.03.048"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124513"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2011.09.014"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9071(200103)63:3<242::AID-JMV1007>3.0.CO;2-N"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040419"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.08.007"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/289373a0"},{"key":"e_1_2_6_32_1","unstructured":"World Health Organization.2011. Antigenic and genetic characteristics of zoonotic influenza viruses and development of candidate vaccine viruses for pandemic preparedness. Available:http:\/\/www.who.int\/influenza\/resources\/documents\/2011_09_h5_h9_vaccinevirusupdate.pdf"},{"key":"e_1_2_6_33_1","unstructured":"World Health Organization.2012a. Cumulative number of confirmed human cases for avian influenza A(H5N1) reported to WHO 2003\u20132012. Available:http:\/\/www.who.int\/influenza\/human_animal_interface\/H5N1_cumulative_table_archives\/en\/index.html"},{"key":"e_1_2_6_34_1","unstructured":"World Health Organization.2012b. Antigenic and genetic characteristics of zoonotic influenza viruses and development of candidate vaccine viruses for pandemic preparedness. Available:http:\/\/www.who.int\/influenza\/vaccines\/virus\/201202_h5_h9_vaccinevirusupdate.pdf"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9820"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002985"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.07142-11"},{"key":"e_1_2_6_38_1","first-page":"104","article-title":"Identification of dual receptor\u2010binding specific strains of human H5N1 viruses in China","volume":"25","author":"Zhou JF","year":"2012","journal-title":"Biomed Environ Sci"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23492","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:20:00Z","timestamp":1700655600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,18]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1002\/jmv.23492"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23492","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,18]]}}}