{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:32:50Z","timestamp":1771461170511,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T00:00:00Z","timestamp":1371081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Routine screening of patients at risk of hepatitis C virus (HCV) infection has become a priority given recent improvements in therapeutic options and the asymptomatic nature of most chronic infections. The aim of this study was to evaluate the performance of the Elecsys\u00ae Anti\u2010HCV II assay, a new qualitative antibody immunoassay, compared with currently available assays, and assess its suitability for routine diagnostic testing. The sensitivity of the Elecsys\u00ae Anti\u2010HCV II, ARCHITECT\u00ae Anti\u2010HCV, AxSYM\u00ae HCV 3.0, PRISM\u00ae HCV, Vitros\u00ae ECi Anti\u2010HCV, Elecsys\u00ae Anti\u2010HCV, and ADVIA Centaur\u00ae HCV assays was compared using commercially available seroconversion panels and samples from patients known to be HCV positive and infected with HCV genotypes 1\u20136. Specificity was investigated using samples from blood donors, unselected hospitalized patients, and patients with potential cross\u2010reacting factors or from high\u2010risk groups. The Elecsys\u00ae Anti\u2010HCV II assay detected more positive bleeds than the comparator assays, was more sensitive in recognizing early HCV infection, and correctly identified all 765 samples known to be HCV positive, regardless of genotype. The overall specificity of the Elecsys<jats:sup>\u00ae<\/jats:sup> Anti\u2010HCV II assay was 99.84% (n\u2009=\u20096,850) using blood donor samples, 99.66% (n\u2009=\u20093,922) using samples from unselected hospitalized patients, and 99.66% (n\u2009=\u20092,397) using samples from patients with potentially cross\u2010reacting factors or from high\u2010risk groups. The specificity of the Elecsys\u00ae Anti\u2010HCV II assay was superior or equal to the comparator assays. In conclusion, the Elecsys\u00ae Anti\u2010HCV II assay is a sensitive and specific assay suitable for routine use in the reliable detection of anti\u2010HCV antibodies. <jats:bold><jats:italic>J. Med. Virol. 85:1362\u20131368, 2013<\/jats:italic><\/jats:bold>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23536","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T19:27:48Z","timestamp":1371151668000},"page":"1362-1368","source":"Crossref","is-referenced-by-count":21,"title":["Multicenter evaluation of the elecsys\u00ae anti\u2010HCV II assay for the diagnosis of hepatitis C virus infection"],"prefix":"10.1002","volume":"85","author":[{"given":"Juan I.","family":"Esteban","sequence":"first","affiliation":[{"name":"Liver Unit Vall d'Hebron University Hospital Barcelona Spain"},{"name":"Networked Biomedical Research Center Hepatic and Digestive Diseases (CIBEREHD) Institute of Health Carlos III Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"van Helden","sequence":"additional","affiliation":[{"name":"Medical Centre Stein and Partner M\u00f6nchengladbach Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flora","family":"Alborino","sequence":"additional","affiliation":[{"name":"Dolo Hospital Laboratory Medicine Local Health Unit 13 Dolo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"B\u00fcrgisser","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Department of Medicine and Laboratory Medicine Centre Hospitalier Universitaire Vaudois Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Cellerai","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Department of Medicine and Laboratory Medicine Centre Hospitalier Universitaire Vaudois Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Pantaleo","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Department of Medicine and Laboratory Medicine Centre Hospitalier Universitaire Vaudois Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Eiras","sequence":"additional","affiliation":[{"name":"Transfusion Centre of Galicia Santiago de Compostela Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria I.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Transfusion Centre of Galicia Santiago de Compostela Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Ghisetti","sequence":"additional","affiliation":[{"name":"Amedeo di Savoia Hospital Laboratory of Microbiology and Virology Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Gleich","sequence":"additional","affiliation":[{"name":"Laboratory Schottdorf Medical Centre GmbH Augsburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Imdahl","sequence":"additional","affiliation":[{"name":"Laboratory Schottdorf Medical Centre GmbH Augsburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Kaiser","sequence":"additional","affiliation":[{"name":"MVZ Laboratory Centre Munich Medical Care Centre Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"M\u00f6ller","sequence":"additional","affiliation":[{"name":"MVZ Laboratory Centre Munich Medical Care Centre Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Wetlitzky","sequence":"additional","affiliation":[{"name":"MVZ Laboratory Centre Munich Medical Care Centre Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Segovia","sequence":"additional","affiliation":[{"name":"Virgen de la Arrixaca Hospital Laboratory Microbiology\/Virology Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Schennach","sequence":"additional","affiliation":[{"name":"Central Institute for Blood Transfusion and Immunology Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelies","family":"M\u00fchlbacher","sequence":"additional","affiliation":[{"name":"Central Institute for Blood Transfusion and Immunology Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,13]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Abbott.2008a. 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