{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:58:05Z","timestamp":1776837485576,"version":"3.51.2"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,12]],"date-time":"2013-04-12T00:00:00Z","timestamp":1365724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High\u2010risk human papillomavirus (HPV) DNA detection provides high sensitivity but low specificity for moderate\u2010grade cervical intraepithelial neoplasia or worse histological identification. A prospective study evaluated mRNA testing efficacy for predicting this histological diagnosis in case of HPV 16 and\/or 18 DNA detection. A total of 165 endocervical samples harboring HPV 16 and\/or 18 DNA were tested with NucliSENS\u2010EasyQ\u00ae HPV E6\/E7\u2010mRNA\u2010assay (Biomerieux, Marcy l\u00b4Etoile, France). Women with cytological alterations were referred to colposcopy (n\u2009=\u2009111). Moderate\u2010grade cervical intraepithelial neoplasia or worse was diagnosed in 25.8% of women presenting atypical squamous cells of undetermined significance or low\u2010grade squamous intraepithelial lesions and in 89.8% of women with high\u2010grade squamous intraepithelial lesions. mRNA sensitivity was 81.3% and 84.1%, respectively. Specificity was 52.2%, and 80.0%, respectively. Negative predictive value (NPV) was 88.9% in undetermined or low\u2010grade squamous lesions. Positive predictive value (PPV) was 97.4% in high\u2010grade squamous lesions. mRNA reduced colposcopies by 44.3% in undetermined or low\u2010grade squamous lesions. Direct treatment of mRNA\u2010positive cases reduced 77.5% of colposcopies in high\u2010grade squamous lesions. Women without cytological alterations were followed for 18 months (n\u2009=\u200935), and moderate\u2010grade cervical intraepithelial neoplasia or worse was diagnosed in 34.3%; mRNA sensitivity and specificity were 83.3% and 86.9%, respectively. PPV and NPV were 76.9% and 90.9%, respectively for predicting moderate\u2010grade cervical intraepithelial neoplasia or worse in 18 months. mRNA reduced the number of visits for follow\u2010up in 62.2%. In conclusion, NucliSENS\u2010EasyQ\u00ae HPV E6\/E7\u2010mRNA\u2010assay (Biomerieux) can serve as a triage test in case of HPV 16 and\/or 18 DNA detection. J. Med. Virol. 85: 1063\u20131068, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmv.23544","type":"journal-article","created":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T16:43:45Z","timestamp":1366044225000},"page":"1063-1068","source":"Crossref","is-referenced-by-count":22,"title":["Human papillomavirus (HPV) E6\/E7 mRNA as a triage test after detection of HPV 16 and HPV 18 DNA"],"prefix":"10.1002","volume":"85","author":[{"given":"Sonia","family":"Perez Castro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amparo","family":"I\u00f1arrea Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Lamas Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Sar\u00e1n 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