{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T11:48:20Z","timestamp":1763466500836},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T00:00:00Z","timestamp":1366156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Financial and operational constraints limit low\u2010resource countries in the screening of high\u2010risk genital human papillomaviruses (HR\u2010HPV), the etiological agents of cervical cancer. With its simple storage, conservation and shipping, dried cervical sample (DCS) could represent an efficient tool. The aim of the study was to evaluate the reliability of HPV genotyping from DCS. Cervical samples were obtained from 50 women infected with HIV\u20101 in C\u00f4te d'Ivoire. After DNA extraction from both DCS and matched liquid cervical samples (LCS), HPV genotyping was performed and the concordance of genotyping results was evaluated. HPV prevalence was 88% in LCS and 78% in DCS. Kappa statistic was 0.51 for the presence of any genotype (95% confidence interval, 0.25\u20130.77) and 0.73 for HR\u2010HPV (0.45\u20130.99). Out of 50 samples, 45 were HPV\u2010positive for DCS and\/or LCS, and HR\u2010HPV were detected in 37 samples (74%) with 36 HR\u2010HPV multiple infections. Any genotype and HR genotype identification was concordant\/compatible in 86% (43\/50) and 88% (44\/50) of samples, respectively. In most instances, kappa statistics for detection of type\u2010specific HPV was over 0.6 (including HPV\u201016, \u201018, \u201031, \u201033). An excellent agreement (kappa statistic\u2009\u2265\u20090.81) was found for eight genotypes (HPV\u20106, \u201031, \u201035, \u201040, \u201056, \u201058, \u201066, and \u201082). In spite of interfering factors (multiple infections, different HPV loads, amplification competition, different inputs), DCS and LCS led to concordant\/compatible results in most cases. DCS could represent an efficient tool for epidemiological field studies in resource\u2010limited settings, and more importantly for improving the screening coverage and care management in women infected with HPV. <jats:bold><jats:italic>J. Med. Virol. 85:1222\u20131228, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23573","type":"journal-article","created":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T19:13:13Z","timestamp":1366225993000},"page":"1222-1228","source":"Crossref","is-referenced-by-count":4,"title":["Dried cervical spots for human papillomaviruses identification"],"prefix":"10.1002","volume":"85","author":[{"given":"Val\u00e9rie","family":"Charbonneau","sequence":"first","affiliation":[{"name":"University Hospital of Bordeaux Virology Laboratory and Biology Department Bordeaux France"},{"name":"University of Bordeaux Fondamental Microbiology and Pathogenicity UMR5234 Bordeaux France"},{"name":"CNRS Fondamental Microbiology and Pathogenicity, UMR5234 Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Garrigue","sequence":"additional","affiliation":[{"name":"University Hospital of Bordeaux Virology Laboratory and Biology Department Bordeaux France"},{"name":"University of Bordeaux Fondamental Microbiology and Pathogenicity UMR5234 Bordeaux France"},{"name":"CNRS Fondamental Microbiology and Pathogenicity, UMR5234 Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Jaquet","sequence":"additional","affiliation":[{"name":"University of Bordeaux, ISPED, Team of HIV, Cancer and Global Health in Africa Centre INSERM U897\u2010Epidemiology\u2010Biostatistics Bordeaux France"},{"name":"INSERM, ISPED, Team of HIV, Cancer and Global Health in Africa Centre INSERM U897\u2010Epidemiology\u2010Biostatistics Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apollinaire","family":"Horo","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics University Hospital of Yopougon Abidjan, Ivory Coast"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Minga","sequence":"additional","affiliation":[{"name":"PAC\u2010CI Program Abidjan, Ivory Coast"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Recordon\u2010Pinson","sequence":"additional","affiliation":[{"name":"University of Bordeaux Fondamental Microbiology and Pathogenicity UMR5234 Bordeaux France"},{"name":"CNRS Fondamental Microbiology and Pathogenicity, UMR5234 Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Dabis","sequence":"additional","affiliation":[{"name":"University of Bordeaux, ISPED, Team of HIV, Cancer and Global Health in Africa Centre INSERM U897\u2010Epidemiology\u2010Biostatistics Bordeaux France"},{"name":"INSERM, ISPED, Team of HIV, Cancer and Global Health in Africa Centre INSERM U897\u2010Epidemiology\u2010Biostatistics Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Fleury","sequence":"additional","affiliation":[{"name":"University Hospital of Bordeaux Virology Laboratory and Biology Department Bordeaux France"},{"name":"University of Bordeaux Fondamental Microbiology and Pathogenicity UMR5234 Bordeaux France"},{"name":"CNRS Fondamental Microbiology and Pathogenicity, UMR5234 Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47520-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/657321"},{"key":"e_1_2_6_4_1","unstructured":"Centers for Disease Control.1992.1993 revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. 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