{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T16:39:49Z","timestamp":1721061589205},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T00:00:00Z","timestamp":1366156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>In vitro studies with primary human pancreatic islets suggest that several enterovirus serotypes are able to infect and replicate in beta cells. Some enterovirus strains are highly cytolytic in vitro whereas others show virus replication with no apparent islet destruction. The capability to induce islet destruction is determined only partially by the virus serotype, since strain specific differences have been detected within some serotypes including echovirus 9 (E\u20109). In this study, the viral genetic factors determining the outcome of islet infection (i.e., destructive vs. benign) were investigated by constructing parallel infectious clones of lytic E\u20109\u2010DM strain that was isolated from a small child at the clinical onset of type 1 diabetes. The capabilities of these clone\u2010derived viruses to induce islet destruction were monitored and the lytic potential of clones was modified by site\u2010directed mutagenesis. The lytic capabilities of these clone\u2010derived viruses in human pancreatic islets were modified by a single amino acid substitution (T81A) in the capsid protein VP1. The data presented outline the importance of amino acid point mutations in the pathogenetic process leading to islet necrosis. However, although the amino acid substitution (T81A) modifies the lytic capabilities of E\u20109\u2010DM strain\u2010derived microvariant strains, it is likely that additional viral genetic determinants of pancreatic islet pathogenicity exist in other E\u20109 strains. <jats:bold><jats:italic>J. Med. Virol. 85:1267\u20131273, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23574","type":"journal-article","created":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T19:13:17Z","timestamp":1366225997000},"page":"1267-1273","source":"Crossref","is-referenced-by-count":12,"title":["A single amino acid substitution in viral VP1 protein alters the lytic potential of clone\u2010derived variants of echovirus 9 DM strain in human pancreatic islets"],"prefix":"10.1002","volume":"85","author":[{"given":"A.","family":"Paananen","sequence":"first","affiliation":[{"name":"Intestinal Viruses Unit National Institute for Health and Welfare Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ylipaasto","sequence":"additional","affiliation":[{"name":"Intestinal Viruses Unit National Institute for Health and Welfare Helsinki 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