{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:52:09Z","timestamp":1760298729729},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Human enterovirus (EV) 71 is the main etiological agent of hand, foot, and mouth disease (HFMD). It is associated with neurological complications, and caused fatalities during recent outbreaks in the Asia\u2013Pacific region. Infections caused by EV71 could lead to many complications, ranging from brainstem encephalitis to pulmonary oedema, resulting in high mortality. In this study, a duplex real\u2010time RT\u2010PCR assay was developed in order to simultaneously detect pan\u2010EV and EV71. EV71\u2010specific primers and probes were designed based on the highly conserved <jats:italic>VP1<\/jats:italic> region of EV71. Five EV71 strains were detected as positive, and no positive fluorescence signal was observed in the duplex real\u2010time RT\u2010PCR for other viral RNA, which showed 100% specificity for the selected panel, and no cross\u2010reactions were observed in this duplex real\u2010time RT\u2010PCR. The EV71\u2010specific duplex real\u2010time RT\u2010PCR was more sensitive than conventional RT\u2010PCR, and detected viral titers that were 10\u2010fold lower than those measured by the latter. Of the 381 HFMD clinical specimens, 196 (51.4%) cases were pan\u2010EV\u2010positive, of which 170 (86.7%) were EV71\u2010positive when tested by pan\u2010EV and EV71\u2010specific duplex real\u2010time RT\u2010PCR. EV71\u2010specific duplex real\u2010time RT\u2010PCR offers a rapid and sensitive method to detect EV71 from clinical specimens, and will allow quarantine measures to be taken more effectively during outbreaks. <jats:bold><jats:italic>J. Med. Virol. 85:1274\u20131279, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23588","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T17:43:18Z","timestamp":1369244598000},"page":"1274-1279","source":"Crossref","is-referenced-by-count":12,"title":["Development of duplex real\u2010time <scp>RT<\/scp>\u2010<scp>PCR<\/scp> based on Taqman technology for detecting simultaneously the genome of pan\u2010enterovirus and enterovirus 71"],"prefix":"10.1002","volume":"85","author":[{"given":"Seoyeon","family":"Hwang","sequence":"first","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byunghak","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyoung","family":"Hong","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahyoun","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyejin","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kisang","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doo\u2010Sung","family":"Cheon","sequence":"additional","affiliation":[{"name":"Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health Korea Centers for Disease Control and Prevention Chungcheongbuk\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(99)00019-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-7722(02)00140-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)74253-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(00)00065-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0904.020395"},{"key":"e_1_2_6_7_1","first-page":"83","volume-title":"A practical approach series, virus culture","author":"Cary J","year":"1999"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.06.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909233411301"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-330"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.4049\/jimmunol.84.3.309","article-title":"Typing of virus by combination of antiserum pools. 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