{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:23:15Z","timestamp":1789773795197,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>This study investigated the role of enteroviruses in sepsis\u2010like illness among neonates in Kuwait. Serum samples from 139 consecutive neonates presenting with sepsis\u2010like illness during a three and a half\u2010year\u2010period whose blood cultures were negative for bacterial pathogens were tested. Enterovirus RNA was detected by single\u2010step reverse\u2010transcription PCR (RT\u2010PCR). Specific genotypes were identified by direct DNA sequencing of enteroviral genome. Serotype\u2010specific antibodies in serum samples from some selected patients were detected by virus neutralization test using coxsackievirus B types (CBVs). All 139 neonates presented with sepsis\u2010like illness and blood samples were uniformly negative for aerobic\/anaerobic bacterial cultures. Fifty\u2010six (40%) neonates had further complications of sepsis including carditis (n\u2009=\u200934) and multi\u2010organ involvement (n\u2009=\u200922). Enterovirus RNA was detected by RT\u2010PCR in 34 of 139 (24%) serum samples which is among the highest frequency reported so far in non\u2010epidemic settings. Genotyping identified CBVs as most common enteroviruses, causing 19 of 34 (56%) enteroviral sepsis episodes in neonates. Of 34 carditis cases, 18 were positive for CBVs by serotyping including all 10 enterovirus RNA\u2010positive samples. Only one fatality was observed due to liver failure in a neonate with hepatitis. Our data showed that enteroviruses are responsible for 24% of neonatal sepsis cases due to non\u2010bacterial causes in Kuwait. The data indicate that enteroviruses should be considered in the differential diagnosis of sepsis\u2010like illness among neonates, particularly those with negative blood cultures for bacterial pathogens. <jats:bold><jats:italic>J. Med. Virol. 85:1280\u20131285, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23604","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T17:43:18Z","timestamp":1369244598000},"page":"1280-1285","source":"Crossref","is-referenced-by-count":19,"title":["Frequency of enterovirus detection in blood samples of neonates admitted to hospital with sepsis\u2010like illness in Kuwait"],"prefix":"10.1002","volume":"85","author":[{"given":"Suhail","family":"Ahmad","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology Kuwait University Safat Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajmal","family":"Dalwai","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology Kuwait University Safat Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Widad","family":"Al\u2010Nakib","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Microbiology Kuwait University Safat Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200108000-00008"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00148581-200406010-00001"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199310000-00005"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200104000-00022"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-002-0929-5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.111.5.964"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.5.843-850.1990"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(85)80663-6"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.10.007"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-7-236"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/599094"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22023"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/339298"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2008.06.008"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00093-07"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1288-1293.1999"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21878"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199910000-00007"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.7.9.16082"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008480-200102000-00012"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(200006)61:2<221::AID-JMV8>3.0.CO;2-E"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31815c1b07"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.23.3.414"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.07.008"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318223bad2"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1086\/589752"},{"key":"e_1_2_5_28_1","first-page":"1","article-title":"Deaths: Final data for 2006","volume":"57","author":"Xu J","year":"2009","journal-title":"Natl Vital Stat Rep"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23604","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T07:44:22Z","timestamp":1700639062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,22]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/jmv.23604"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23604","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,22]]}}}