{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:12:16Z","timestamp":1761394336004},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T00:00:00Z","timestamp":1371081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Primary infection with varicella zoster virus (VZV) occurs in immunocompromised and immunocompetent individuals. Clinical and asymptomatic reactivation with shedding of infectious virus and viremia may occur. The prevalence of VZV viremia is unknown. The aim of this study was to detect VZV viremia and quantify VZV DNA using quantitative polymerase chain reaction (qPCR) in blood from different populations. A qPCR\u2010based method using EvaGreen\u00ae was used to quantify VZV DNA in 491 samples, including whole blood, plasma and buffy\u2010coat, from patients hospitalized with varicella\u2010associated disease (Group 1, n\u2009=\u200910) and three groups with no VZV disease: individuals with a first clinical diagnosis of central nervous system demyelination (Group 2, n\u2009=\u2009213) with their age and sex\u2010matched controls (Group 3, n\u2009=\u2009218); and HIV\u2010infected individuals (Group 4, n\u2009=\u200950). VZV\u2010specific IgG antibody titres were measured in Group 3. The proportion positive for viremia and mean detectable VZV DNA load (copies\/ml) were: Group 1: 100% (10\/10) and 4.6\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009\u00b1\u20091.4\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>; Group 2: 4% (9\/213) and 1.5\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u2009\u00b1\u20091.8\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>; Group 3: 8% (17\/218) and 1.1\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u2009\u00b1\u20097.8\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>; Group 4: 12% (6\/50) and 7.7\u2009\u00d7\u200910<jats:sup>1<\/jats:sup>\u2009\u00b1\u20092.8\u2009\u00d7\u200910<jats:sup>2<\/jats:sup>. VZV DNA load and IgG titres were not significantly correlated (Group 3 only). VZV load in Group 1 was significantly elevated compared to Groups 2\u20134 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001); the latter were not significantly different from each other (<jats:italic>P<\/jats:italic>\u2009=\u20090.05). VZV genotypes from clades 1\u20135 were identified in Group 1. VZV DNA was detected but at low frequency and viral load in both immunocompetent and immunocompromised individuals asymptomatic for VZV infection, compared to individuals with active VZV infection. <jats:bold><jats:italic>J. Med. Virol. 85:1491\u20131497, 2013<\/jats:italic><\/jats:bold>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23605","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T19:27:48Z","timestamp":1371151668000},"page":"1491-1497","source":"Crossref","is-referenced-by-count":9,"title":["Varicella zoster virus quantitation in blood from symptomatic and asymptomatic individuals"],"prefix":"10.1002","volume":"85","author":[{"given":"Cheryl S.","family":"Toi","sequence":"first","affiliation":[{"name":"Clinical Virology, Centre for Infectious Diseases and Microbiology\u2014Public Health and Laboratory Services, Institute of Clinical Pathology and Medical Research Westmead Hospital and University of Sydney Westmead NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meav\u2010Lang J.","family":"Lay","sequence":"additional","affiliation":[{"name":"Clinical Virology, Centre for Infectious Diseases and Microbiology\u2014Public Health and Laboratory Services, Institute of Clinical Pathology and Medical Research Westmead Hospital and University of Sydney Westmead NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robyn","family":"Lucas","sequence":"additional","affiliation":[{"name":"National Centre for Epidemiology and Population Health College of Medicine, Biology and Environment, The Australian National University Canberra ACT Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choo Beng","family":"Chew","sequence":"additional","affiliation":[{"name":"Clinical Virology, Centre for Infectious Diseases and Microbiology\u2014Public Health and Laboratory Services, Institute of Clinical Pathology and Medical Research Westmead Hospital and University of Sydney Westmead NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janette","family":"Taylor","sequence":"additional","affiliation":[{"name":"Clinical Virology, Centre for Infectious Diseases and Microbiology\u2014Public Health and Laboratory Services, Institute of Clinical Pathology and Medical Research Westmead Hospital and University of Sydney Westmead NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Louise","family":"Ponsonby","sequence":"additional","affiliation":[{"name":"Murdoch Childrens Research Institute The Royal Children's Hospital Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic E.","family":"Dwyer","sequence":"additional","affiliation":[{"name":"Clinical Virology, Centre for Infectious Diseases and Microbiology\u2014Public Health and Laboratory Services, Institute of Clinical Pathology and Medical Research Westmead Hospital and University of Sydney Westmead NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"The Ausimmune Investigator Group (AIG)","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(02)00037-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.Supplement_1.S35"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.017814-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.5.1153"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.2.314"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21614"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2568-2573.2000"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1995.00430150071008"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-5-15"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200003023420906"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.5.1264"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458507077174"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e31820af93d"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.6.1292"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199809)56:1<91::AID-JMV15>3.0.CO;2-Z"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20777"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.950-953.1999"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.478"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2008.03.011"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20253"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00874-07"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.53202"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000024920"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21316"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-006-0402-x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.03.027"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.019547-0"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199510000-00006"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2009.08.037"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1565-1568.2003"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23605","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T18:38:28Z","timestamp":1698691108000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,13]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1002\/jmv.23605"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23605","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2013,6,13]]}}}