{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T08:11:00Z","timestamp":1698739860015},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T00:00:00Z","timestamp":1371081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>Current screening methods for uterine cervical cancer such as Papanicolaou smears and\/or high risk human Papillomavirus (HR\u2010HPV) detection have a high negative predictive value but a low positive predictive value for the presence of high grade cervical lesions. Therefore, new parameters are needed to reduce the rate of unnecessary referrals for colposcopy. The predictive value of the HPV multiplex ligation\u2010dependent probe amplification (MLPA) assay, which can assess simultaneously HPV16\/18 viral load and viral integration, was evaluated. The assay was applied to 170 cervical cytological samples, and the results were correlated with the matching histological follow\u2010up. The GP5+\/6+ assay and qPCR were used as a control for HR\u2010HPV typing. The MLPA assay classified a higher percentage of cases as high\u2010risk (high\u2010viral load and\/or viral integration) with higher grades of dysplasia. There was a high correlation between the HPV MLPA assay and qPCR for viral load and HPV genotyping, and between the MLPA assay and the GP5+\/6+ assay for HPV genotyping. The sensitivity and specificity of the HPV MLPA assay for the detection of high\u2010grade lesions were 44% and 93%, respectively. This study demonstrates that the HPV MLPA assay can reliably detect HPV 16\/18, viral load, and viral integration in cytological samples. Also, high\u2010risk classification correlated well with the presence of high\u2010grade dysplasia. However, for the implementation of the MLPA assay into clinical practice, additional HR\u2010HPV types need to be included to increase the sensitivity of the assay, and thereby increase its negative predictive value. <jats:bold><jats:italic>J. Med. Virol. 85:1386\u20131393, 2013<\/jats:italic><\/jats:bold>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23629","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T19:27:48Z","timestamp":1371151668000},"page":"1386-1393","source":"Crossref","is-referenced-by-count":4,"title":["Use of the HPV MLPA assay in cervical cytology for the prediction of high grade lesions"],"prefix":"10.1002","volume":"85","author":[{"given":"Rogier J.N.T.M.","family":"Litjens","sequence":"first","affiliation":[{"name":"Department of Molecular Cell Biology, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center Maastricht The Netherlands"},{"name":"Department of Obstetrics and Gynecology, GROW\u2010School for Oncology and Developmental Biology Maastricht University Medical Center Maastricht The 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