{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:21:09Z","timestamp":1787311269269,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,7,12]],"date-time":"2013-07-12T00:00:00Z","timestamp":1373587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      In this study, real\u2010time PCR was used to quantify adenovirus DNA in cell suspensions prepared from 106 right and left tonsils and 10 adenoids obtained from 57 patients who underwent routine tonsillectomies and\/or adenoidectomies. Eighty\u2010four (72.4%) tonsils and adenoids samples were positive for HAdV by real\u2010time PCR. The viral load ranged from 2.8\u2009\u00d7\u200910\n                      <jats:sup>2<\/jats:sup>\n                      to 2.6\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      \u2009copies\/10\n                      <jats:sup>7<\/jats:sup>\n                      cells and varied up to sixfold between the right and left tonsils. In some cases, only one tonsil was positive and the viral load was lower in older children. Seventy\u2010eight of 84 positive samples could be typed by sequencing of the hexon L1 region. Species C (types 1, 2, and 5) were detected in 84.1% of the patients followed by types 3 and 7 of species B (6.8%), HAdV\u2010E4 (6.8%), and HAdV\u2010F41 (2.3%). In one patient adenovirus C2 was found in the left tonsil and adenovirus C5 in the right tonsil. No DNA methylation was detected in either the E1A promoter or the major late promoter region of adenovirus DNA from six tonsils and adenoids samples and two clinical isolates.\n                      <jats:bold>\n                        <jats:italic>J Med. Virol. 85:1947\u20131954, 2013<\/jats:italic>\n                        .\n                      <\/jats:bold>\n                      . \u00a9 2013 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.23678","type":"journal-article","created":{"date-parts":[[2013,7,12]],"date-time":"2013-07-12T12:07:18Z","timestamp":1373630838000},"page":"1947-1954","source":"Crossref","is-referenced-by-count":23,"title":["Prevalence and quantitation of adenovirus DNA from human tonsil and adenoid tissues"],"prefix":"10.1002","volume":"85","author":[{"given":"Moustafa Alissa","family":"Alkhalaf","sequence":"first","affiliation":[{"name":"Virology Unit, Institute of Inflammation and Repair The University of Manchester Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm","family":"Guiver","sequence":"additional","affiliation":[{"name":"Health Protection Agency Manchester United 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