{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:52:43Z","timestamp":1753275163175},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T00:00:00Z","timestamp":1376006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Myelitis is one of the rarest neurological complications of varicella zoster virus (VZV) infection. In this study, the authors remodeled the \u201cwide\u2010range\u201d quantitative nested real\u2010time (QNRT) polymerase chain reaction (PCR) assay to quantitatively detect a small amount of VZV\u2010DNA in cerebrospinal fluid (CSF). For use as a specific internal control \u201ccalibrator,\u201d an original mutation\u2010VZV (MZ) plasmid was developed. The initial copy number of VZV\u2010DNA in CSF specimens was measured by the amplification rate of the MZ\u2010plasmid. For 17 consecutive CSF specimens collected from three elderly patients with VZV myelitis, the diagnostic value of the wide\u2010range QNRT\u2010PCR assay was evaluated and compared with other conventional PCR assays and enzyme immunoassay (EIA). The MZ\u2010plasmid demonstrated statistically uniform amplifications (<jats:italic>F<\/jats:italic>\u2009=\u20091.016) against a wide range (1\u2013100,000) of copy numbers of mimic VZV\u2010DNA. The wide\u2010range QNRT\u2010PCR assay quantitatively and rapidly (within 48\u2009hr) detected 5,863, 3,052, 958, and 6,721\u2009copies\/ml of VZV\u2010DNA in the CSF specimens collected from all patients in the acute phase. Additionally, there was a significant difference (*<jats:italic>P<\/jats:italic>\u2009=\u20090.023) in the copy number of VZV\u2010DNA between before and after acyclovir treatment. Other conventional single PCR assays all revealed negative results, but were nevertheless time\u2010consuming (7 days). The IgG EIA\u2010value for VZV was continually elevated throughout the clinical course of all patients. The MZ\u2010plasmid was thus regarded as an appropriate \u201ccalibrator\u201d in the wide\u2010range QNRT\u2010PCR assay. This assay is a novel, rapid, accurate, quantitative, and highly sensitive technique, and will contribute as a reliable and useful clinical examination for the rapid diagnosis of VZV infection to central nervous system. <jats:bold><jats:italic>J Med. Virol. 85:2042\u20132055, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23690","type":"journal-article","created":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T21:45:33Z","timestamp":1376084733000},"page":"2042-2055","source":"Crossref","is-referenced-by-count":6,"title":["Diagnostic value of a \u201cwide\u2010range\u201d quantitative nested real\u2010time PCR assay for varicella zoster virus myelitis"],"prefix":"10.1002","volume":"85","author":[{"given":"Teruyuki","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Neurology Nagaoka\u2010Nishi Hospital Nagaoka Japan"},{"name":"Department of Neurology Hino Municipal Hospital Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Neurology Nagaoka\u2010Nishi Hospital Nagaoka Japan"},{"name":"Division of Neurology Department of Medicine Nihon University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Takasu","sequence":"additional","affiliation":[{"name":"Department of Neurology Nagaoka\u2010Nishi Hospital Nagaoka Japan"},{"name":"Division of Neurology Department of Medicine Nihon University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822011000400026"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822011000600026"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.47.4.929"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/114.3.1181"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430403"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00141-07"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.44.10.1818"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00153-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2006.090316"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2010.01.019"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1973.00490290049004"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.5692\/clinicalneurol.50.634"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3274-3279.2000"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.44.6.1161"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4426-4432.2001"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1352-1355.1999"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/37.7.1153"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199508000-00010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.20.13070-13081.2005"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.1029-1039.2006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000162052.13838.B2"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2007.01.071"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02214-07"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01200-07"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803008999"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-008-0982-6"}],"container-title":["Journal 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