{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T09:44:47Z","timestamp":1721382287570},"reference-count":63,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T00:00:00Z","timestamp":1376870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Human herpesviruses (HHVs) are involved in the pathogenesis of different types of uveitis. Cytokine response plays an important role in virus\u2010induced immunopathology. This study aimed to investigate the incidence of HHVs in aqueous humor samples of immunocompetent patients with suspected viral uveitis and cytokine (IL\u201010, IL\u20106, and IFN\u2010\u03b3) expression profiling. Forty\u2010seven aqueous humor samples were collected from immunocompetent patients with viral uveitis. Samples were assayed for HHV\u20101 to HHV\u20108 by in\u2010house real\u2010time polymerase chain reactions. IL\u20106, IL\u201010, and IFN\u2010\u03b3 were quantified with a cytometric bead array. Relations between viral detection, cytokine profiles, and clinical data were studied. At least one viral genome was detected in 21 aqueous humor samples analyzed. Varicella\u2010zoster virus (VZV) was detected in 14 of the positive samples, cytomegalovirus (CMV) in 8, HSV\u20101 in 1, Epstein\u2013Barr virus (EBV) in 4, and HHV\u20106 in 2. More than one viral genome was detected in seven aqueous humor samples. Aqueous humor samples positive for HHV\u2010DNA contained significant levels of IL\u20106, IL\u201010, and IFN\u2010\u03b3, compared to HHV\u2010DNA negative samples. High levels of IL\u20106 were detected in patients with CMV\u2010DNA in their aqueous humor samples. Significantly higher levels of IL\u201010 and IFN\u2010\u03b3 were found in positive samples for VZV, EBV, and HHV\u20106 DNA than in negative aqueous humor ones. VZV was the principal etiologic agent of uveitis in this Tunisian series, with CMV the second most common agent. Knowledge of immunoregulatory interactions and dynamic changes in viral uveitis may be a key to understand the pathogenesis leading to more\u2010effective treatments. <jats:bold><jats:italic>J. Med. Virol. 85:2079\u20132086, 2013<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23708","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T22:24:47Z","timestamp":1376951087000},"page":"2079-2086","source":"Crossref","is-referenced-by-count":11,"title":["Herpesvirus detection and cytokine levels (IL\u201010, IL\u20106, and IFN\u2010\u03b3) in ocular fluid from Tunisian immunocompetent patients with uveitis"],"prefix":"10.1002","volume":"85","author":[{"given":"Imen","family":"Nahdi","sequence":"first","affiliation":[{"name":"Laboratory of Contagious Diseases and Biologically Active Substances, LR99\u2010ES27 Faculty of Pharmacy, Monastir University Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rym Ben","family":"Abdelwahed","sequence":"additional","affiliation":[{"name":"Laboratory of Contagious Diseases and Biologically Active Substances, LR99\u2010ES27 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