{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T05:43:38Z","timestamp":1772516618934,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T00:00:00Z","timestamp":1382572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"VLIR-UOS collaboration between the University of Limpopo (South Africa) and University of Antwerp (Belgium)"},{"DOI":"10.13039\/501100001323","name":"Poliomyelitis Research Foundation (PRF)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001323","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research Foundation (NRF) (both from South Africa)"},{"name":"(to R.L.L); Canon Collins Educational Trust, NRF-DAAD, Stella and Paul Leowestein Charitable and Educational Trust."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:sec><jats:label\/><jats:p>This study reports the detection of HPV types from cancerous and pre\u2010cancerous penile lesions that were diagnosed histologically. Sixty\u2010six (22 pre\u2010cancerous and 44 cancerous lesions) tissue biopsies, received between 2004 and 2011 by the Anatomical Pathology Department at Dr. George Mukhari Hospital were selected for this study. Total DNA was extracted and genotyped using type specific real\u2010time quantitative polymerase chain reaction (qPCR) for 18 HPV types. Of 66 samples, only 51 were included in the analysis. Overall, HPV 11 (50.9%) and HPV 16 (49.1%) showed almost similar incidence in the study patients. In pre\u2010cancerous lesions, HPV 11 was more frequent (80.0%), followed by HPV 31 and HPV 16 at 25.0% each and other HPV types included 35 (15.0%), 59 (15.0%), 53 (10.0%), 33 (10.0%), 18 (5.0%), 51 (5.0%), 52 (5.0%), 56 (5.0%), and 67 (5.0%). For cancerous lesions, HPV 16 was the most detected (62.9%), followed by HPV 11 (34.3%), and other HPV types included 18 (11.4%), 33 (5.7%), 39 (5.7%), 45 (5.7%), 66 (5.7%), 52 (2.9%), 58 (2.9%), 6 (2.9%), and 67 (2.9%). Several lesions demonstrated multiple HPV infections, ranging from two to six different types in one lesion. The study showed high diversity of HPV types in cancerous and pre\u2010cancerous lesions of South African males with the most frequent being HPV types 11 and 16. The data suggest that boys could directly benefit from vaccination as they are exposed to variety of HPV types as early as 10 years of age in Africa. <jats:bold><jats:italic>J. Med. Virol. 86:257\u2013265, 2014<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23730","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T08:56:53Z","timestamp":1382605013000},"page":"257-265","source":"Crossref","is-referenced-by-count":19,"title":["Diversity of HPV types in cancerous and pre\u2010cancerous penile lesions of South African men: Implications for future HPV vaccination strategies"],"prefix":"10.1002","volume":"86","author":[{"given":"Ramokone L.","family":"Lebelo","sequence":"first","affiliation":[{"name":"Department of Virology University of Limpopo, Medunsa Campus and National Health Laboratory Service Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ga\u00eblle","family":"Boulet","sequence":"additional","affiliation":[{"name":"Applied Molecular Biology Research Group University of Antwerp Groenenborgerlaan Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelius M.","family":"Nkosi","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology University of Limpopo, Medunsa Campus Pretoria South Africa"},{"name":"National Health Laboratory Service Dr. George Mukhari Tertiary Laboratory Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mechack N.","family":"Bida","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology University of Limpopo, Medunsa Campus Pretoria South Africa"},{"name":"National Health Laboratory Service Dr. George Mukhari Tertiary Laboratory Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John\u2010Paul","family":"Bogers","sequence":"additional","affiliation":[{"name":"Applied Molecular Biology Research Group University of Antwerp Groenenborgerlaan Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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