{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:16:12Z","timestamp":1740104172985,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:00:00Z","timestamp":1384819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81171549"],"award-info":[{"award-number":["81171549"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Hepatitis E virus (HEV) is a hepatotropic virus that causes acute and, occasionally, chronic viral hepatitis. At least four recognized genotypes of mammalian HEV have been identified. Genotypes 3 and 4 are zoonotic in humans and pigs are the major host. The 7.2\u2009kb genome of HEV contains three open reading frames: ORF1, ORF2, and ORF3. ORF1 encodes a nonstructural protein and the ORF2 protein is the main capsid protein but the precise functions of ORF3 protein remain obscure. To explore the role of ORF3 in the porcine host, the genotype 4 ORF3 protein was used for yeast two\u2010hybrid screening to find cellular binding partners encoded by a pig liver cDNA library, two porcine liver specific proteins, plasminogen and \u03b12\u2010antiplasmin were identified. Their interactions with the ORF3 protein were validated by chemiluminescent Co\u2010Immunoprecipitation assay and by Western blotting in Co\u2010IP and His pull\u2010down assay. The biological significance of these interactions and their possible role in the HEV infection are discussed. <jats:bold><jats:italic>J. Med. Virol. 86:487\u2013495, 2014<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23800","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T11:47:57Z","timestamp":1384861677000},"page":"487-495","source":"Crossref","is-referenced-by-count":3,"title":["Hepatitis E virus open reading frame 3 protein interacts with porcine liver\u2010specific plasminogen and \u03b12\u2010antiplasmin"],"prefix":"10.1002","volume":"86","author":[{"given":"Yan","family":"Zhou","sequence":"first","affiliation":[{"name":"Division of HIV\/AIDS and Sex\u2010Transmitted Virus Vaccines National Institutes for Food and Drug Control Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansheng","family":"Geng","sequence":"additional","affiliation":[{"name":"Health Science Center Hebei University Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Surgery St Jude Children's Research Hospital Memphis Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of HIV\/AIDS and Sex\u2010Transmitted Virus Vaccines National Institutes for Food and Drug Control Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim J.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Division of Medicine University College London Medical School London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youchun","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of HIV\/AIDS and Sex\u2010Transmitted Virus Vaccines National Institutes for Food and Drug Control Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22493"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq085"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.12.2889"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00403-08"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0064-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01994-09"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0903778"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.08.001"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00593-10"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056320"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH11-08-0601"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.0030214"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21474"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.02.050"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02571-08"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32830ee08a"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101546200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.023804-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2741\/Lijnen"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.482"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2009.01257.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/250464"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01340.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21116"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.035378-0"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.02.002"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.6.2005"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi7016552"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.009274-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310964200"},{"key":"e_1_2_6_32_1","first-page":"36p","volume-title":"Fundamentals of pathology: Medical course and step 1 review, first edition","author":"Sattar HA","year":"2011"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501691102"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509568200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402017200"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.010561-0"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.12.9045-9053.1997"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1086\/504293"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23800","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23800","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T15:49:46Z","timestamp":1696348186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23800"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,19]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1002\/jmv.23800"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23800","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2013,11,19]]}}}