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At the present time, RSV infection is diagnosed by immunofluorescence, test pack and\/or PCR, obtaining better results with PCR than with any other technique. The production of new monoclonal antibodies (mAbs) capable of detecting RSV in clinical samples is necessary to generate better and faster diagnosis tools for RSV. In this study, three new mAbs, directed against the RSV N and M2\u20101 proteins, were evaluated for the detection of RSV in clinical samples. Nasopharyngeal swabs were obtained from: 27 RSV\u2010positive patients; 15 human metapneumovirus (hMPV)\u2010positive patients; and 6 healthy controls. To evaluate RSV presence in these samples, clinical samples and RSV\u2010infected cells were tested by Enzyme\u2010Linked ImmunoSorbent Assay (ELISA), flow cytometry, immunofluorescence, and dot\u2010blot assays. Specificity and sensitivity were determined for each mAb by using purified RSV antigens and antigens from different viruses. Infected cells and clinical samples tested with the three new mAbs resulted positive by immunofluorescence, ELISA, flow cytometry, and dot blot. No false positives were obtained in samples infected with other respiratory virus (hMPV) or from healthy controls. These results suggest that these new anti\u2010RSV mAbs can be considered for the rapid and reliable detection of RSV on infected cells and clinical specimens by multiple immunological approaches. <jats:bold><jats:italic>J. Med. Virol. 86:1256\u20131266, 2014<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23807","type":"journal-article","created":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T05:46:46Z","timestamp":1382420806000},"page":"1256-1266","source":"Crossref","is-referenced-by-count":16,"title":["Respiratory syncytial virus detection in cells and clinical samples by using three new monoclonal antibodies"],"prefix":"10.1002","volume":"86","author":[{"given":"Roberto S.","family":"G\u00f3mez","sequence":"first","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Departamento de Gen\u00e9tica Molecular y Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas Pontificia Universidad Cat\u00f3lica de Chile Santiago Chile"},{"name":"INSERM U1064 Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"Mora","sequence":"additional","affiliation":[{"name":"Millennium 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