{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T09:11:06Z","timestamp":1787994666899,"version":"build-2784847793"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T00:00:00Z","timestamp":1392249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:label\/><jats:p>Influenza A\/H3N2 viruses are associated with severe epidemics. Antiviral resistance and continued antigenic drift are the major concerns regarding prophylaxis and treatment of influenza. The objectives of this study were to investigate the prevalence and frequency of antiviral drug resistance in influenza A\/H3N2 viruses circulating among Shanghainese children from June 2009 to May 2012 and to understand the genetic evolution of the hemagglutinin (HA) epitopes. Nasopharyngeal\/throat swabs were collected from outpatients with influenza\u2010like illness. Of the 3,475 children tested, 344 (9.9%) were positive for influenza A\/H3N2 viruses. Epidemics of influenza A\/H3N2 occurred in July\u2013September 2009, August 2010\u2013January 2011, and November 2011\u2013May 2012. The 71 A\/H3N2\u2010positive specimens were sequenced to characterize the genotypic antiviral resistance and genetic drift in the HA epitopes. All of the 71 A\/H3N2 viruses sequenced were genotypically resistant to adamantine but sensitive to oseltamivir. The HA1 sequence analysis revealed that the A\/H3N2 viruses underwent constant mutations in the HA antigenic sites over the three seasons compared with the corresponding vaccine strains, and amino acid changes in at least three epitopes were observed each season. Phylogenic analyses indicated that the A\/H3N2 strains circulating in Shanghai fell into clades different from those of the corresponding seasonal vaccine strains and were grouped into the A\/Perth\/16\/2009 genetic clade and the A\/Victoria\/208\/2009 genetic clades 3B, 3C, and 5. The continuous monitoring of genetic drift and antiviral resistance of influenza viruses is important for the management of influenza and for updating the vaccine composition. <jats:bold><jats:italic>J. Med. Virol. 86:1026\u20131033, 2014<\/jats:italic>.<\/jats:bold> \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23854","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T13:52:16Z","timestamp":1392299536000},"page":"1026-1033","source":"Crossref","is-referenced-by-count":5,"title":["Prevalence, genetic drift of haemagglutinin, and antiviral resistance of influenza A\/H3N2 viruses circulating in Shanghai in children during 2009\u20132012"],"prefix":"10.1002","volume":"86","author":[{"given":"Jiehao","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangshi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baihui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Microbiology Department Shanghai Municipal Center for Disease Control and Prevention Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilei","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.09.016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.11.043"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/508777"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67338-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181ff863b"},{"key":"e_1_2_6_7_1","unstructured":"Chinese National Influenza Center.(CNIC).2009. Chinese Influenza Weekly Report (Week 48: Nov 30\u2013Dec 6 2009)."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/518936"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2009.00103.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.10.011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2011.07.015"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318257172d"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/18.Supplement_B.19"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-S1-S31"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP1656"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01993-08"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.03.026"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02288-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.210.21"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000321358"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.03.014"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00555-08"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm103"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00366-3"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.86.287"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.11.1333"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp038238"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.043059-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis1220"},{"key":"e_1_2_6_32_1","unstructured":"World Health Organization (WHO).2012. Influenza Centre London.February 2012 interim report. Report prepared for the WHO annual consultation on the composition of influenza vaccine for the Northern Hemisphere. 20th\u201322nd February 2012."},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/289373a0"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.003513"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196909112811102"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis628"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP1538"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.23854","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.23854","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T16:07:03Z","timestamp":1696349223000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.23854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,13]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/jmv.23854"],"URL":"https:\/\/doi.org\/10.1002\/jmv.23854","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,13]]}}}