{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:18:24Z","timestamp":1792009104351,"version":"4.4.0"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T00:00:00Z","timestamp":1388361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Dengue virus (DENV) is the causative agent of dengue and severe dengue. To understand better the dengue virus\u2010host interaction, it is important to determine how the expression of cellular proteins is modified due to infection. Therefore, a comparison of protein expression was conducted in Vero cells infected with two different DENV strains, both serotype 2: DENV\u20102\/NG (associated with dengue) and DENV\u20102\/16681 (associated with severe dengue). The viability of the infected cells was determined, and neither strain induced cell death at 48\u2009hr. In addition, the viral genomes and infectious viral particles were quantified, and the genome of the DENV\u20102\/16681 strain was determined to have a higher replication rate compared with the DENV\u20102\/NG strain. Finally, the proteins from infected and uninfected cultures were separated using two\u2010dimensional gel electrophoresis, and the differentially expressed proteins were identified by mass spectrometry. Compared with the uninfected controls, the DENV\u20102\/NG\u2010 and DENV\u20102\/16681\u2010infected cultures had five and six differentially expressed proteins, respectively. The most important results were observed when the infected cultures were compared to each other (DENV\u20102\/NG vs. DENV\u20102\/16681), and 18 differentially expressed proteins were identified. Based on their cellular functions, many of these proteins were linked to the increase in the replication efficiency of DENV. Among the proteins were calreticulin, acetyl coenzyme A, acetyl transferase, and fatty acid\u2010binding protein. It was concluded that the infection of Vero cells with DENV\u20102\/NG or DENV\u20102\/16681 differentially modifies the expression of certain proteins, which can, in turn, facilitate infection. <jats:bold><jats:italic>J. Med. Virol. 86:1448\u20131458, 2014<\/jats:italic>.<\/jats:bold> \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.23857","type":"journal-article","created":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T09:55:24Z","timestamp":1388397324000},"page":"1448-1458","source":"Crossref","is-referenced-by-count":16,"title":["Infection of epithelial cells with dengue virus promotes the expression of proteins favoring the replication of certain viral strains"],"prefix":"10.1002","volume":"86","author":[{"given":"Viviana","family":"Mart\u00ednez\u2010Betancur","sequence":"first","affiliation":[{"name":"Programa de Estudio y Control de Enfermedades Tropicales\u2010PECET Universidad de Antioquia Medellin Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Mar\u00edn\u2010Villa","sequence":"additional","affiliation":[{"name":"Programa de Estudio y Control de Enfermedades Tropicales\u2010PECET Universidad de Antioquia Medellin Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marl\u00e9n","family":"Mart\u00ednez\u2010Gutierrez","sequence":"additional","affiliation":[{"name":"Programa de Estudio y Control de Enfermedades Tropicales\u2010PECET Universidad de Antioquia Medellin Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2006.0562"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(81)90142-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0897-866"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02575-06"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.12.013"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1295-1304.2006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03194597"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031082"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.10.003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.16.7769-7773.2001"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000086"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2190"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.71.652"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65124"},{"key":"e_1_2_6_17_1","first-page":"276","article-title":"Observations related to the pathogenesis of dengue hemorrhagic fever. 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