{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:42:27Z","timestamp":1789724547887,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,8,2]],"date-time":"2014-08-02T00:00:00Z","timestamp":1406937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/8\/7\/17\/2010-ECD-1"],"award-info":[{"award-number":["5\/8\/7\/17\/2010-ECD-1"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:label\/><jats:p>Human parvovirus B19 (B19V) has been associated with chronic anemia in immuno\u2010compromised patients. In the present study, the prevalence and genotype distribution of B19V in children from North India, suffering with hemato\u2010oncological disorders is reported. Children with aplastic anemia\/leukemia\/chronic hematological disorders, and healthy blood donors were enrolled in the study. Blood samples from cases and blood donors were analyzed for anti\u2010B19V IgM and anti\u2010B19V IgG antibodies by ELISA and for B19V\u2010DNA by PCR. B19V\u2010DNA positive samples were studied further for determination of viral load in samples and for B19V\u2010DNA sequence (VP1\/VP2 overlapping region) analysis. Total 238 cases (103 leukemia, 77 aplastic anemia and 58 chronic hematological disorders) and 350 blood donors were enrolled in the study. Anti\u2010B19V IgM was positive in 16 (6.7%) cases, B19V\u2010DNA was detected in 13 (5.5%) cases and anti\u2010B19V IgG was positive in 127 (53.4%) cases. Total 223 (63.5%) blood donors were positive for anti\u2010B19V IgG, however, anti\u2010B19V IgM and B19V\u2010DNA was not detected in any blood donor. The prevalence of anti\u2010B19V IgG was significantly higher in children &gt; 10 years of age. Viral load of B19V decreased with appearance of specific antibodies. Phylogenetic analysis of the VP1\/VP2 overlapping region revealed that genotype 1 predominated in these patients (11\/13, 84.6%), followed by genotype 3 (2\/13, 15.4%). No genotype 2 was detected. All the genotype 1strains were sub\u2010typed as 1a, except four strains, which matched neither 1a nor 1b and formed a separate cluster. Both the genotype 3 strains were sub\u2010typed as 3b. <jats:bold><jats:italic>J. Med. Virol. 87:303\u2013309, 2015<\/jats:italic>.<\/jats:bold> \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24028","type":"journal-article","created":{"date-parts":[[2014,8,2]],"date-time":"2014-08-02T07:59:38Z","timestamp":1406966378000},"page":"303-309","source":"Crossref","is-referenced-by-count":30,"title":["Prevalence and genotypic characterization of human parvovirus B19 in children with hemato\u2010oncological disorders in North India"],"prefix":"10.1002","volume":"87","author":[{"given":"Parul","family":"Jain","sequence":"first","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amita","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danish N","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desh D","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Paediatrics King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmalya R","family":"Moulik","sequence":"additional","affiliation":[{"name":"Department of Paediatrics King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulika","family":"Chandra","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.4.105"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00292-5"},{"key":"e_1_2_6_4_1","first-page":"139","article-title":"A pilot study on the seroprevalence of parvovirus B19 infection","volume":"115","author":"Abraham M","year":"2002","journal-title":"Indian J Med Res"},{"key":"e_1_2_6_5_1","first-page":"580","volume-title":"Essential paediatrics","author":"Arya LS","year":"2009"},{"key":"e_1_2_6_6_1","first-page":"1484","article-title":"Human parvovirus 4 as Potential cause of Encephalitis in children","volume":"17","author":"Benjamin LA","year":"2011","journal-title":"India. 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