{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:23:48Z","timestamp":1761765828536},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,3]],"date-time":"2014-09-03T00:00:00Z","timestamp":1409702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:label \/><jats:p>The criterion standard for the diagnosis of occult hepatitis C virus (HCV) infection is detection of HCV\u2010RNA in liver cells. However, because of the invasive nature of liver biopsy, other methods have been studied<jats:bold>.<\/jats:bold> The present study aimed to identify subjects with occult HCV\u20104 infection among healthy sexual partners of patients with chronic HCV\u20104 infection by detecting HCV\u2010RNA in peripheral blood mononuclear cells (PBMCs) using real\u2010time polymerase chain reaction (PCR). Fifty healthy Egyptian spouses of patients with chronic HCV\u20104 infection were included in this study. Real\u2010time PCR was used to detect HCV\u2010RNA in PBMCs in all the study subjects. The prevalence of occult HCV\u20104 infection was 4%, and a statistically significant higher prevalence was found among patients with a history of sexually transmitted infection. The results of the present study indicate the importance of intra\u2010spousal transmission of HCV\u20104 infection, especially in subjects with a history of sexually transmitted infection. <jats:bold><jats:italic>J. Med. Virol. 87:424\u2013427, 2015<\/jats:italic>.<\/jats:bold> \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24074","type":"journal-article","created":{"date-parts":[[2014,9,3]],"date-time":"2014-09-03T10:36:08Z","timestamp":1409740568000},"page":"424-427","source":"Crossref","is-referenced-by-count":14,"title":["Detection of occult hepatitis C virus among healthy spouses of patients with HCV infection"],"prefix":"10.1002","volume":"87","author":[{"given":"Yahia El","family":"Shazly","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaled","family":"Hemida","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona","family":"Rafik","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reham Al","family":"Swaff","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zainab A.","family":"Ali\u2010Eldin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaimaa","family":"GadAllah","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Medicine Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,3]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"127","article-title":"Hepatitis C virus inter\u2010spousal transmission could be a gate to reduce intra\u2010familial spread and high prevalence of HCV in Egypt","volume":"77","author":"Abou ElAzm AR","year":"2009","journal-title":"Med. 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