{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:16Z","timestamp":1786978096689,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,6]],"date-time":"2015-05-06T00:00:00Z","timestamp":1430870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Viral reactivations following hematopoietic stem cell transplantation are thought to result from the breakdown of both cell\u2010mediated and humoral immunity. As a result, many viruses could be reactivated individually or simultaneously. Using a multiplex polymerase chain reaction (PCR), we prospectively examined many kinds of viral DNAs at a time in 105 patients who underwent allogeneic hematopoietic stem cell transplantation. In total, 591 whole blood samples were collected weekly from pre\u2010 to 42 days post\u2010transplantation and the following 13 viruses were tested; herpes simplex virus 1 (HSV\u20101), HSV\u20102, varicella\u2010zoster virus (VZV), Epstein\u2013Barr virus (EBV), cytomegalovirus (CMV), human herpes virus 6 (HHV\u20106), HHV\u20107, HHV\u20108, adenovirus, BK virus (BKV), JC virus (JCV), parvovirus B19, and hepatitis B virus (HBV). Several viral DNAs were detected in 12 patients before hematopoietic stem cell transplantation. The detection rate gradually increased after transplantation and peaked at 21 days. The most frequently detected virus was HHV\u20106 (n\u2009=\u200963; 60.0%), followed by EBV (n\u2009=\u200911; 10.5%), CMV (n\u2009=\u200911; 10.5%), and HHV\u20107 (n\u2009=\u20099; 8.6%). Adenovirus and HBV were each detected in one patient (1.0%). Detection of HHV\u20106 DNA was significantly more common among patients undergoing cord blood transplantation or with steroid treatment. EBV DNA tended to be more common in patients treated with anti\u2010thymocyte globulin. Multiplex PCR was useful for detecting many viral reactivations after hematopoietic stem cell transplantation, simultaneously. Cord blood transplantation, steroid treatment, or anti\u2010thymocyte globulin use was confirmed to be risk factors after transplantation.\n                      <jats:bold>\n                        <jats:italic>J. Med. Virol. 87:1427\u20131435, 2015<\/jats:italic>\n                        .\n                      <\/jats:bold>\n                      \u00a9 2015 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.24161","type":"journal-article","created":{"date-parts":[[2015,5,6]],"date-time":"2015-05-06T10:58:24Z","timestamp":1430909904000},"page":"1427-1435","source":"Crossref","is-referenced-by-count":40,"title":["Large\u2010scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation"],"prefix":"10.1002","volume":"87","author":[{"given":"Natsuko","family":"Inazawa","sequence":"first","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsukasa","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Yoto","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Nojima","sequence":"additional","affiliation":[{"name":"Department of Public health Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Hokkaido Medical Center for Child Health and Rehabilitation Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Virology Medical Research Institute Tokyo Medical and Dental University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,6]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"777","article-title":"Human herpes virus\u20106 infection in marrow graft recipients: Role in pathogenesis of graft\u2010versus\u2010host disease. 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